Unknown,Transcriptomics,Genomics,Proteomics

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Identification of targets regulated by SELP in HSC


ABSTRACT: To understand the underlying mechanism by which Alox15 gene is required by HSCs, we performed a comparative DNA microarray analysis using total RNA isolated from wild type Lin-Sca-1+c-Kit+, SELP-/- Lin-Sca-1+c-Kit+. The result was validated by quantitative real-time PCR analysis of wild type Lin-Sca-1+c-Kit+ and SELP-/- Lin-Sca-1+c-Kit+. Cancer stem cells are responsible for the initiation and maintenance of some types of cancer, and few effective target genes in these stem cells have been identified. Here we show that the selp is essential for the survival of leukemia stem cells (LSCs) in BCR-ABL-induced chronic myeloid leukemia (CML). To understand the underlying mechanism through which SELP regulates the function of HSCs, the gene expression profiles between WT and Selp-/- HSCs were compared. To understand the underlying mechanism through which SELP regulates the function of HSCs, the gene expression profiles between WT and Selp-/- HSCs were compared.

ORGANISM(S): Mus musculus

SUBMITTER: yaoyu chen 

PROVIDER: E-GEOD-57459 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival.

Chen Yaoyu Y   Peng Cong C   Abraham Sheela A SA   Shan Yi Y   Guo Zhiru Z   Desouza Ngoc N   Cheloni Giulia G   Li Dongguang D   Holyoake Tessa L TL   Li Shaoguang S  

The Journal of clinical investigation 20140808 9


Cancer stem cells (CSCs) are responsible for the initiation and maintenance of some types of cancer, suggesting that inhibition of these cells may limit disease progression and relapse. Unfortunately, few CSC-specific genes have been identified. Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of leukemia stem cells (LSCs) in a murine model of BCR-ABL-induced chronic myeloid leukemia (CML). In the absence of Alox15, BCR-ABL was  ...[more]

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