Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

64 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of LSD2-depleted HepG2 cells

ABSTRACT: Transcriptome analysis of LSD2-depleted HepG2 cells revealed that many of the target genes were related to lipid metabolism. We found that LSD2 is an important epigenetic regulator of hepatic lipid metabolism. We depleted LSD2 in HepG2 human hepatic cells using three different siRNAs, and then carried out an expression microarray experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Shinjiro Hino

PROVIDER: E-GEOD-56645 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

ChIP-seq analysis of LSD2/H3K4me1 interaction in control and LSD2-knockdowned HepG2 cells

Project description:ChIP-seq analysis of LSD2-depleted HepG2 cells revealed that many of the target genes were related to lipid metabolism. We found that LSD2 is an important epigenetic regulator of hepatic lipid metabolism. Examination of LSD2/H3K4me1 interaction in control and LSD2-knockdowned HepG2 cells.

2015-02-02 | E-GEOD-59830 | biostudies-arrayexpress

ChIP-seq analysis of LSD2/H3K4me1 interaction in control and LSD2-knockdowned HepG2 cells

2015-02-02 | GSE59830 | GEO

Transcriptome analysis of LSD2-depleted HepG2 cells

Project description:Transcriptome analysis of LSD2-depleted HepG2 cells revealed that many of the target genes were related to lipid metabolism. We found that LSD2 is an important epigenetic regulator of hepatic lipid metabolism.

2015-02-02 | GSE56645 | GEO

ChIP-seq analysis of LSD2/DNA interaction in HepG2 cells in normal condition

Project description:ChIP-seq analysis of HepG2 cells revealed that many of the target genes of LSD2 were related to lipid metabolism. We found that LSD2 is an important epigenetic regulator of hepatic lipid metabolism. Examination of LSD2/DNA interaction in HepG2 cells in normal condition.

2015-02-02 | E-GEOD-59694 | biostudies-arrayexpress

ChIP-seq analysis of LSD2/DNA interaction in HepG2 cells in normal condition

2015-02-02 | GSE59694 | GEO

Role of histone lysine demethylase LSD2 in hepatic metabolism

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-31 | E-GEOD-59695 | biostudies-arrayexpress

Role of histone demethylase LSD1 in hepatic cancer metabolism

Project description:Transcriptome analysis of LSD1-depleted HepG2 cells revealed that LSD1 regulates the expression of glycolytic and mitochondrial metabolism genes. We found that LSD1 is an important regulator of glycolysis and mitochondrial respiration in hepatoma. We depleted LSD1 in HepG2 human hepatoma cells using two different siRNAs, and then carried out an expression microarray experiment.

2015-05-21 | E-GEOD-69075 | biostudies-arrayexpress

Homo sapiens

Project description:Transcriptome analysis of LSD2-depleted HepG2 cells

| PRJNA244154 | ENA

Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating H3K4me2 methylation (Affymetrix)

Project description:Dynamic histone H3K4 methylation is an important epigenetic component of transcriptional regulation. However, most of our current understanding of this histone mark is confined to regulation of transcriptional initiation. We now show that human LSD2/KDM1b/AOF1, the human homolog of LSD1, is a novel H3K4me1/2 demethylase that specifically regulates histone H3K4 methylation within intragenic regions of its target genes. Genome-wide mapping reveals that LSD2 associates predominantly with the gene bodies of actively transcribed genes, but is markedly absent from promoters. Depletion of endogenous LSD2 results in an increase of H3K4me2 as well as a decrease of H3K9me2 at LSD2 binding sites, and a consequent dysregulation of target gene transcription. Furthermore, characterization of LSD2 complex revealed that LSD2 forms active complexes with euchromatic histone methyltransferases EHMT1/2 and NSD3 as well as cellular factors involved in active transcription elongation. These data provide a possible molecular mechanism linking LSD2 to transcriptional regulation post initiation. We used microarray analysis to identify the subset of genes that are differentially expression after depletion of endogenous LSD2/KDM1b/AOF1.

2010-11-01 | GSE22859 | GEO

Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation (ChIP-chip)

2010-11-01 | GSE22870 | GEO