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Systems analysis of the response of photosynthesis, metabolism and growth to an increase in irradiance in the photosynthesis model organism Chlamydomonas reinhardtii

ABSTRACT: Systems analysis reveals that Chlamydomonas reinhardtii responds rapidly and flexibly to an increase in light intensity. Rising metabolite levels and post-translation regulation facilitate a rapid increase in the rate of carbon fixation and a slightly delayed increase in the rate of growth, and slower changes in protein abundance adjust allocation and minimize bottlenecks in the new conditions. Gene expression was measured from samples of Chlamydomonas reinhardtii cell cultures at four time points (two under low, four under high light) under either low (41 Âµmol) or high (145 Âµmol) light conditions in two separate bioreactors. Three biological replicate time series were sampled.

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Samuel Arvidsson

PROVIDER: E-GEOD-56800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systems analysis of the response of photosynthesis, metabolism and growth to an increase in irradiance in the photosynthesis model organism Chlamydomonas reinhardtii

Project description:Systems analysis reveals that Chlamydomonas reinhardtii responds rapidly and flexibly to an increase in light intensity. Rising metabolite levels and post-translation regulation facilitate a rapid increase in the rate of carbon fixation and a slightly delayed increase in the rate of growth, and slower changes in protein abundance adjust allocation and minimize bottlenecks in the new conditions.

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Proteomics and constraint-based modelling reveal enzyme kinetic properties of C. reinhardtii on a genome-scale

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Comparison of the photoprotection mechanism in Chlorella Ohadii and Chlamydomonas reinhardtii

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Expression analysis of Chlamydomonas reinhardtii exposed to silver

Project description:Chlamydomonas reinhardtii exposed to various concentrations of silver For this experiment,C. reinhardtii were exposed to (4) different concentrations of silver, as biological triplicates

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Oxidative and electrophilic stress conditions and the sor1 mutant

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Acetate and light stress timecourse expression profiling in C. reinhardtii

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Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival

Project description:Here we show that the phytochrome-less chlorophyte Chlamydomonas reinhardtii retains a functional pathway to synthesize the linear tetrapyrrole (bilin) precursor of the phytochrome chromophore. Reverse genetic, metabolic inactivation and bilin rescue experiments establish that this pathway is needed for heme iron acquisition and for the diurnal transition to phototrophic growth. RNA-Seq measurements reveal a bilin-dependent signaling network that is necessary for the heterotrophic to phototrophic transition. These results imply the presence of a novel bilin sensor pathway that may be widely distributed amongst oxygenic photosynthetic organisms. We isolated RNA from heterotrophic suspension cultures of 4A+ WT and the hmox1 mutant grown in the presence or absence of 0.1 mM BV IXM-NM-1 before and after transfer to low light.

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Transcriptome analysis of Chlamydomonas reinhardtii chloroplast and mitochondria

Project description:Here, we report on the transcriptome of the organelles of the micro-alga, Chlamydomonas reinhardtii, sampled under a number of different conditions. The preparation of the RNA-Seq libraries and their analysis were performed using protocols optimized for organellar transcripts. Samples include growth in media +/– Fe, growth in media +/– Cu, diurnal growth samples collected in dark and light, and the sexual cycle.

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The small, high-light-induced protein SliP4 is involved in the proper organization of photosynthetic complexes in thylakoid membranes of Synechocystis sp. PCC 6803

Project description:An increasing number of small proteins has been identified in the genomes of well-annotated model organisms including photosynthetic cyanobacteria such as Synechocystis sp. PCC 6803. A newly assigned protein comprising 37 amino acids was analyzed that is encoded upstream of the super-oxide dismutase SodB encoding gene. The initial hypothesis that this small protein might be functionally related to SodB could not be supported, instead we provide evidence that it fulfills important functions related to the organization of photosynthetic complexes. Therefore, we named it small light-induced protein of 4 kDa, SliP4. SliP4 is strongly induced under high-light-conditions and it is co-localized to photosystem I and II as well as NDH1 complexes on thylakoid membranes. Tagged SliP4 was used to purify NDH1 complexes, where it is especially associated with the subunits NdhF1 and NdhD1. Mutation of the protein-coding part in the sliP4 gene resulted in a high-light-sensitive phenotype, because the mutant was not further able to induce cyclic electron flow and state transitions under high-light conditions. Collectively our data indicate that SliP4 contributes to the association of NDH1 with the photosystem I and/or photosystem II complexes to facilitate different electron transfer modes under high light conditions.

2023-06-07 | PXD036788 | Pride

UNASSAMBLED CELL WALL PROTEINS FORM AGGREGATES IN THE EXTRACELLULAR SPACE OF CHLAMYDOMONAS REINHARDTII STRAIN UVM4

Project description:The green microalga Chlamydomonas reinhardtii is emerging as a promising cell biofactory for secreted recombinant protein (RP) production. In recent years, the generation of the broadly-used cell wall-deficient mutant strain UVM4 has allowed for a drastic increase in secreted RP yields. However, purification of secreted RPs from the extracellular space of C. reinhardtii strain UVM4 is challenging. Previous studies suggest that secreted RPs are trapped in a matrix of cell wall protein aggregates populating the secretome of strain UVM4, making it difficult to isolate and purify the RPs. To better understand the nature and behaviour of these extracellular protein aggregates, we analysed and compared the extracellular proteome of the strain UVM4 to its cell-walled ancestor, C. reinhardtii strain 137c. When grown under the same conditions, strain UVM4 produced a unique extracellular proteomic profile, including a higher abundance of secreted cell wall glycoproteins. Further characterization of high molecular weight extracellular protein aggregates in strain UVM4 revealed that they are largely comprised of pherophorins, a specific class of cell wall glycoproteins. Our results offer important new insights into the extracellular space of strain UVM4, including strain-specific secreted cell wall proteins and the composition of the aggregates possibly related to impaired RP purification. The discovery of pherophorins as a major component of extracellular protein aggregates will inform future strategies to remove or prevent aggregate formation, enhance purification of secreted RPs, and improve yields of recombinant biopharmaceuticals in this emerging cell biofactory.

2022-08-11 | PXD032195 | Pride

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