Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profile of human cardiac fibroblasts (HCFs), induced cardiomyocytes (iCMs), and heart

ABSTRACT: Global gene expression profile of total 24460 probes in the iCMs. The gene expression shifts from a fibroblast state toward a cardiac-like phenotype by Gata4/Mef2c/Tbx5/Mesp1/Myocd (GMTMM) or GMTMM/miR-133 transduction at 7 days after transduction. MiR-133 silenced fibroblast signatures in parallel with cardiac gene activation, and Snai1 overexpression inhibited the effects of miR-133-mediated cardiac reprogramming. HCFs were used for negative control, human heart tissue for positive control. Gene expression profiles were compared among HCFs, iCMs and heart. 24460 probes were analyzed in each experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Satoshi Kondo 

PROVIDER: E-GEOD-56874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Fibroblasts can be directly reprogrammed into cardiomyocyte-like cells (iCMs) by overexpression of cardiac transcription factors or microRNAs. However, induction of functional cardiomyocytes is inefficient, and molecular mechanisms of direct reprogramming remain undefined. Here, we demonstrate that addition of miR-133a (miR-133) to Gata4, Mef2c, and Tbx5 (GMT) or GMT plus Mesp1 and Myocd improved cardiac reprogramming from mouse or human fibroblasts by directly repressing Snai1, a master regulat  ...[more]

Similar Datasets

Transcriptomics Gene expression profiles of mouse embryonic fibroblasts (MEFs), induced cardiomyocytes (iCMs) and heart
2014-07-03 | E-GEOD-56875 | biostudies-arrayexpress
Transcriptomics Gene expression profiles of induced cardiomyocytes(iCMs)
2014-07-03 | E-GEOD-56913 | biostudies-arrayexpress
Transcriptomics Circulating microRNA profiles in patients with type-1 autoimmune hepatitis
2016-08-01 | E-GEOD-71432 | biostudies-arrayexpress
Transcriptomics The change of mRNA expression in murine immortalized podocyte after miR-26a silencing
2014-10-01 | E-GEOD-51210 | biostudies-arrayexpress
Transcriptomics Identification of genes differentially expressed in lung cancer cells after the transfection of miR-221 or miR-222.
2015-05-22 | E-GEOD-57676 | biostudies-arrayexpress
Transcriptomics IL7R: WT vs mutant in vitro and in vivo.
2014-01-08 | E-GEOD-51211 | biostudies-arrayexpress
Transcriptomics miR-487b, miR-3963 and miR-6412 delayed myogenic differentiation in C2C12 mouse myoblast-derived cells.
2015-11-24 | E-GEOD-63454 | biostudies-arrayexpress
Transcriptomics Gene expression profiles of mouse embryonic fibroblasts (MEFs), induced cardiomyocytes (iCMs) and heart
2014-07-03 | GSE56875 | GEO
Transcriptomics Gene expression profile of human cardiac fibroblasts (HCFs), induced cardiomyocytes (iCMs), and heart
2014-07-03 | GSE56874 | GEO
Transcriptomics Gene expression analysis of synovial sarcoma, clinical samples
2015-07-23 | E-GEOD-65532 | biostudies-arrayexpress