Unknown,Transcriptomics,Genomics,Proteomics

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Expression data for T47D cells treated with 2mM hydroxyurea

ABSTRACT: RAD51B, a paralog of RAD51, have been associated with breast cancer risk in genome-wide association studies. The underlying biological mechanism through which germline genetic variation in RAD51B confers susceptibility to breast cancer is not well understood. Here we investigate the molecular function of RAD51B in breast cancer cell lines. We used microarrays to detail the global gene expression to identify classes of genes that are regulated differnetly post DNA damages as a result of RAD51B depeletion. T47D cells were first transfected with either non-targeting (control; siCON) or RAD51B-targeting (experimental; siRAD51B) siRNA, then followed by 24hr treatment of 2mM hydroxyurea (HU). Total RNA were extracted at the end of the treatment for microarray analysis. Three biological replicates were carried out for both control and experimental samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Lea Jessop 

PROVIDER: E-GEOD-56940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RAD51B Activity and Cell Cycle Regulation in Response to DNA Damage in Breast Cancer Cell Lines.

Lee Phoebe S PS   Fang Jun J   Jessop Lea L   Myers Timothy T   Raj Preethi P   Hu Nan N   Wang Chaoyu C   Taylor Philip R PR   Wang Jianjun J   Khan Javed J   Jasin Maria M   Chanock Stephen J SJ  

Breast cancer : basic and clinical research 20141012

Common genetic variants mapping to two distinct regions of RAD51B, a paralog of RAD51, have been associated with breast cancer risk in genome-wide association studies (GWAS). RAD51B is a plausible candidate gene because of its established role in the homologous recombination (HR) process. How germline genetic variation in RAD51B confers susceptibility to breast cancer is not well understood. Here, we investigate the molecular function of RAD51B in breast cancer cell lines by knocking down RAD51B  ...[more]

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