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The Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [Mnase-seq]

ABSTRACT: The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex MNase digestion of purified DNA followed by paired-end sequencing was performed in order to investigate genome-wide nucleosomal positioning in S. cerevisiae under multiple experimental conditions.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Lidia Lopez-Serra

PROVIDER: E-GEOD-56992 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions.

Lopez-Serra Lidia L Kelly Gavin G Patel Harshil H Stewart Aengus A Uhlmann Frank F

Nature genetics 20140831 10

The cohesin complex is at the heart of many chromosomal activities, including sister chromatid cohesion and transcriptional regulation. Cohesin loading onto chromosomes depends on the Scc2-Scc4 cohesin loader complex, but the chromatin features that form cohesin loading sites remain poorly understood. Here we show that the RSC chromatin remodeling complex recruits budding yeast Scc2-Scc4 to broad nucleosome-free regions, which the cohesin loader helps to maintain. Consequently, inactivation of e ...[more]

PMID: 25173104

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