4sUDRB-seq: measuring transcription elongation and initiation genomewide
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Gilad Fuchs
PROVIDER: E-GEOD-57116 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
4sUDRB-seq: measuring genomewide transcriptional elongation rates and initiation frequencies within cells.
Fuchs Gilad G Voichek Yoav Y Benjamin Sima S Gilad Shlomit S Amit Ido I Oren Moshe M
Genome biology 20140509 5
Although transcriptional elongation by RNA polymerase II is coupled with many RNA-related processes, genomewide elongation rates remain unknown. We describe a method, called 4sUDRB-seq, based on reversible inhibition of transcription elongation coupled with tagging newly transcribed RNA with 4-thiouridine and high throughput sequencing to measure simultaneously with high confidence genome-wide transcription elongation rates in cells. We find that most genes are transcribed at about 3.5 Kb/min, w ...[more]
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