Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression Profiles in Chronic Idiopathic Urticaria

ABSTRACT: Lesions of chronic idiopathic urticaria (CIU) showed significant up-regulation of 506 genes and reduced expression of 51 genes. Those most up-regulated are predominantly involved in cell adhesion (e.g. SELE), cell activation (e.g. CD69), and chemotaxis (e.g. CCL2). Twelve independent canonical pathways with p ≤ 0.001 were identified (including intracellular kinase pathways (RAN and JAK/Interferon), cytokine signaling pathways (IL-9, IL10, and IFN), a strong inflammatory response (interferon, IL-9, IL-10, iNOS, and glucocorticoid pathways) and increased cell proliferation (RAN signaling, cell cycle control, and tRNA charging). In addition, cellular functions associated with viral infections were dramatically up-regulated (activation z-score= 5.1; p = 4.97 X 10^-10). We enrolled 8 patients with CIU and 6 healthy controls and obtained 4mm punch biopsies of active lesions and unaffected skin of patients with CIU and of skin from normal controls. Routine histologic evaluation was performed, RNA was isolated and gene expression data was determined using an Affymetrix Human Gene 1.0 ST Array (www.affymetrix.com). Due to technical reasons, the final evaluation included 6 samples of lesional skin, 7 samples of non-lesional skin and 5 samples of normal skin.

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Dreskin

PROVIDER: E-GEOD-57178 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The autoinflammatory disorder, Neonatal-onset Multisystem Inflammatory Disease (NOMID) is the most severe phenotype of disorders caused by mutations in the CIAS1 gene that result in increased production and secretion of active IL-1≤. NOMID patients present with systemic and organ-specific inflammation of the skin, central nervous system and bony growth plates, and respond dramatically to treatment with IL-1 blocking agents. We compared the cellular infiltrates and transcriptome of skin biopsies from patients with NOMID (n=14) before treatment [lesional (LS) and non-lesional (pre-NL) skin] and after treatment (post-NL) with the IL-1 blocker anakinra (recombinant IL-1 receptor antagonist, Kineret®), to normal skin (n=5). Abundant neutrophils distinguish LS skin from pre-NL and post-NL skin. CD11c+ dermal dendritic cells and CD163+ macrophages expressed activated caspase-1 and are the likely sources of cutaneous IL-1 production. Treatment with anakinra led to the disappearance of neutrophils, but CD3+ T cells and HLA-DR+ cells remained elevated. Among the upregulated genes IL-6, IL-8, TNF, IL-17A, CCL20, and the neutrophil defensins DEFA1 and DEFA3 were differentially regulated in LS tissues (compared to normal skin). Important significantly downregulated pathways included IL-1R/TLR signaling, type I and II cytokine receptor signaling, mitochondrial dysfunction, and antigen presentation. Differential expression and regulation of microRNAs and pathways involved in post-transcriptional modification are indicative of epigenetic modification in the various tissue states. Overall, the dysregulated genes and pathways suggest extensive “adaptive” mechanisms to control inflammation, and maintain tissue homeostasis in the skin of patients with NOMID. To determine the transcsriptome of skin samples in Neonatal-onset Multisystem Inflammatory Disease (NOMID), using pre-treatment non-lesional (pre-NL, n=4); lesional (LS, n=6); post-treatment non-lesional (post-NL, n=8), and normal skin (n=5). Comparison of mean gene expression of each group at baseline, and considering treatment effect

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Gene Expression Profiles in Chronic Idiopathic Urticaria

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