Dataset Information

Microdialysis analysis of common inflammatory skin diseases highlights abnormalities in nonlesional skin of patients with atopic dermatitis

ABSTRACT: Insight into the pathophysiology of inflammatory skin diseases, especially at the proteomic level, is severely hampered by the lack of adequate in situ data. Skin microdialysis samples from patients with atopic dermatitis (AD, n=6), psoriasis vulgaris (PSO, n=7) or prurigo nodularis (PN, n=6), as well as healthy controls (n=7) were subjected to proteomics and multiplex cytokine analysis. Single-cell RNA sequencing of skin biopsy specimens was used to identify the cellular origin of cytokines. Among the top 20 enriched GO annotations, NAD metabolic process, regulation of secretion by cell, and pyruvate metabolic process were elevated in microdialysates from lesional AD skin compared with both nonlesional skin and controls. The top 20 enriched KEGG pathways in these three groups overlapped almost completely. In contrast, nonlesional skin from patients with PSO or PN and control skin showed no overlap with lesional skin in this KEGG pathway analysis. Lesional skin from patients with PSO, but not AD or PN, showed significantly elevated protein levels of IL-22 and MCP-1 compared to nonlesional skin. IL-8 was elevated in lesional vs nonlesional AD and PSO skin, whereas IL-12p40 was higher only in lesional PSO skin. Integrated single-cell RNA-seq data revealed identical cellular sources of these cytokines in AD, PSO and PN. Based on microdialysate, proteomic data of lesional PSO and PN skin, but not lesional AD skin, differed significantly from those of nonlesional skin. IL-8, IL-22, MCP-1 and IL-12p40 might be suitable markers for minimally invasive molecular profiling.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

DISEASE(S): Atopic Dermatitis,Psoriasis,Itching

SUBMITTER: Christof Lenz

LAB HEAD: Christof Lenz

PROVIDER: PXD050245 | Pride | 2024-08-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20240229_ProteinID_and_Quant.xlsx	Xlsx
	T_Buhl_240619_010220_DIA_A_09.wiff	Wiff
	T_Buhl_240619_010220_DIA_A_09.wiff.scan	Wiff
	T_Buhl_240619_010220_DIA_A_11.wiff	Wiff
	T_Buhl_240619_010220_DIA_A_11.wiff.scan	Wiff

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Publications

Skin microdialysis detects distinct immunologic patterns in chronic inflammatory skin diseases.

Hollstein Moritz Maximilian MM Traidl Stephan S Heetfeld Anne A Forkel Susann S Leha Andreas A Alkon Natalia N Ruwisch Jannik J Lenz Christof C Schön Michael Peter MP Schmelz Martin M Brunner Patrick P Steinhoff Martin M Buhl Timo T

The Journal of allergy and clinical immunology 20240812 6

<h4>Background</h4>Insight into the pathophysiology of inflammatory skin diseases, especially at the proteomic level, is severely hampered by the lack of adequate in situ data.<h4>Objective</h4>We characterized lesional and nonlesional skin of inflammatory skin diseases using skin microdialysis.<h4>Methods</h4>Skin microdialysis samples from patients with atopic dermatitis (AD, n = 6), psoriasis vulgaris (PSO, n = 7), or prurigo nodularis (PN, n = 6), as well as healthy controls (n = 7), were su ...[more]

PMID: 39142443

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