Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Crucial genes and pathways in chicken germ stem cell differentiation

ABSTRACT: The male germ cell differentiation is a subtle and complex regulation processes, currently its regulatory mechanism is not fully understood. In our experiment, we performed the first comprehensive genome wide analyses of the crucial genes in three kinds of crucial cells (ESCs, PGCs and SSCs) associated with the male germ cells differentiation. We identified thousands of differentially expressed genes (DEGs) in this process andwe choosed 173 candidate genes involved in the differentiation and metabolic processes, like GAL9, AMH, PLK1, PSMD7 and so on. Further exploration found that the candidate genes express patterns were the same between in vitro induction experiments and transcriptome results. Our results clues to the mechanistic basis of male germ cell differentiation and provides an important reference for future studies. Gene expression in chicken germ stem cells was measured at different stages of development. Three independent experiments were performed at each stage (ESCs, PGCs and SSCs).

ORGANISM(S): Gallus gallus

SUBMITTER: zhentao zhang 

PROVIDER: E-GEOD-57213 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Male germ cell differentiation is a subtle and complex regulatory process. Currently, its regulatory mechanism is still not fully understood. In our experiment, we performed the first comprehensive genome and transcriptome-wide analyses of the crucial genes and signaling pathways in three kinds of crucial cells (embryonic stem cells, primordial germ cell, and spermatogonial stem cells) that are associated with the male germ cell differentiation. We identified thousands of differentially expresse  ...[more]

Similar Datasets

Transcriptomics Crucial genes and pathways in chicken germ stem cell differentiation
2015-06-17 | GSE57213 | GEO
Genomics H3K4me2 Chromatin Immunoprecipitation sequencing analysis of chicken Embryonic stem cells, Primordial germ cells and Spermatogonial stem cells
2020-03-21 | GSE147255 | GEO
Proteomics Molecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells
2021-11-03 | PXD022452 | Pride
Transcriptomics Integrative analysis of the acquisition of pluripotency in PGCs
2015-04-08 | E-GEOD-67616 | biostudies-arrayexpress
Transcriptomics An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (in vitro, in vivo)
2013-12-31 | E-GEOD-39546 | biostudies-arrayexpress
Transcriptomics An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl knockdown)
2013-12-31 | E-GEOD-39535 | biostudies-arrayexpress
Transcriptomics An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl testis RNA-IP)
2013-12-31 | E-GEOD-39545 | biostudies-arrayexpress
Transcriptomics An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl native RNA-IP)
2013-12-31 | E-GEOD-39544 | biostudies-arrayexpress
Transcriptomics An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl UV RNA-IP)
2013-12-31 | E-GEOD-39541 | biostudies-arrayexpress
Proteomics Multiomics Approach to Profiling Development of the Spermatogonial Stem Cell Niche Focused on Sertoli cell Maturation
2023-02-24 | PXD037764 | Pride