Unknown,Transcriptomics,Genomics,Proteomics

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Gene induction in mouse C57BL6 kidney cortex by angiotensin converting enzyme inhibition (captopril)


ABSTRACT: Attempt to identify genes whose expression changed in the kidney cortex with angiotensin converting enzyme inhibition. Eleven week-old male C57BL6 mice were treated with captopril at 10mg/kg/day in drinking water for 7 days. The kidney cortex was surgically excised and total RNA was isolated using Trizol (Invitrogen) from three treated and three control mice and was further purified using RNeasy MinElute Cleanup spin columns (Qiagen) according to manufacturer’s instructions. Probes generated from the resulting RNA were hybridized to Illumina Expression BeadChips (mouseWG-6_V2).

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Reudelhuber 

PROVIDER: E-GEOD-57239 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

General lysosomal hydrolysis can process prorenin accurately.

Xa Lucie K LK   Lacombe Marie-Josée MJ   Mercure Chantal C   Lazure Claude C   Reudelhuber Timothy L TL  

American journal of physiology. Regulatory, integrative and comparative physiology 20140625 5


Renin, an aspartyl protease that catalyzes the rate-limiting step of the renin-angiotensin system, is first synthesized as an inactive precursor, prorenin. Prorenin is activated by the proteolytic removal of an amino terminal prosegment in the dense granules of the juxtaglomerular (JG) cells of the kidney by one or more proteases whose identity is uncertain but commonly referred to as the prorenin-processing enzyme (PPE). Because several extrarenal tissues secrete only prorenin, we tested the hy  ...[more]

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