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Mouse kidney cortex: control vs. low sodium diet + captopril treatment

ABSTRACT: miRNA profiling of mouse kidney cortex comparing control vs. low sodium diet + captopril treatment to induce renin expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE31779 | GEO | 2012/02/25

SECONDARY ACCESSION(S): PRJNA145287

REPOSITORIES: GEO

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Mouse kidney cortex: control vs. low sodium diet + captopril treatment

Project description:miRNA profiling of mouse kidney cortex comparing control vs. low sodium diet + captopril treatment to induce renin expression. Two condition experiment: control vs treated; biological replicates: individual mice - 3 control, 3 treated. One replicate per array.

2012-02-25 | E-GEOD-31779 | biostudies-arrayexpress

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Gene expression profiles of renin producing cells in the adult kidney (captopril treated animals) isolated from Renin-YFP transgenic mice using FACS (GUDMAP Series ID:46).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Renin-YFP transgenic mice were utilized to isolate renin producing cells from the kidneys of Adult mice treated with captopril, which was provided in the drinking water at a concentration of 0.5mg/ml. In addition, mice were given low-salt chow. Mice were treated for two weeks before renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

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Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC)

Project description:We report the changes in miRNA expression in the renal cortex od the PCK rats fed diets with different sodium content

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Microarray gene expression profiling of aorta genes of APOE-deficient mice receiving the ACE inhibitor captopril

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Project description:Mouse kidney cortex: control vs. low sodium diet + captopril treatment

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Heart Failure, DITPA and Captopril

Project description:Three weeks after infarction, age matched rats with large infarction by ECG criteria were randomly assigned to one of the three groups: 1) Treatment with Captopril for 21 days and a daily subcutaneous dose of DITPA for the last 10 days of treatment, 2) Treatment for 10 days with DITPA alone, 3) Control heart failure and 4) Sham-operated. Keywords: Drug Effects on Heart Failure

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