Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of alternative splicing in Zea mays: landscape and genetic regulation


ABSTRACT: Analysis of the maize alternative splicing landscape, including transcript discovery and mapping of genotype-dependent variations in alternative splicing using B73, Mo17 and the SX19 inbred mapping population Total RNA was isolated from 5 week old leaves of hydroponically grown maize plants and used to construct RNA seq libraries

ORGANISM(S): Zea mays

SUBMITTER: Shawn Thatcher 

PROVIDER: E-GEOD-57337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of alternative splicing in Zea mays: landscape and genetic regulation.

Thatcher Shawn R SR   Zhou Wengang W   Leonard April A   Wang Bing-Bing BB   Beatty Mary M   Zastrow-Hayes Gina G   Zhao Xiangyu X   Baumgarten Andy A   Li Bailin B  

The Plant cell 20140923 9


Alternative splicing enhances transcriptome diversity in all eukaryotes and plays a role in plant tissue identity and stress adaptation. To catalog new maize (Zea mays) transcripts and identify genomic loci that regulate alternative splicing, we analyzed over 90 RNA-seq libraries from maize inbred lines B73 and Mo17, as well as Syn10 doubled haploid lines (progenies from B73 × Mo17). Transcript discovery was augmented with publicly available data from 14 maize tissues, expanding the maize transc  ...[more]

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