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ChIP-seq profiling of H3K27 tri-methylation in Ezh2-deficient and control murine lung epithelium at day E16.5

ABSTRACT: Epithelial (CD31-CD45-EpCAM+) lung cells were derived by FACS from ShhCre;Ezh2fl/fl and control ShhCre;Ezh2fl/+ mouse embryos at day E16.5 (2 biological replicates per genotype). Chromatin from each of the replicates was immunoprecipitated with H3K27me3 antibody (Millipore #07-449) and subjected to NGS library preparation using TruSeq Nano DNA Sample Preparation Kit (Illumina). Completed libraries from different samples were sequenced on HiSeq 2500 TruSeq with SBS Kit v3 - HS reagents (Illumina) as 100 bp single end reads at the Australian Genome Research Facility. ChIP-seq profiles of H3K27 tri-methylation from Ezh2 deficient and control lung epithelium (2 replicates per genotype).

ORGANISM(S): Mus musculus

SUBMITTER: Marie-Liesse Asselin-Labat

PROVIDER: E-GEOD-57392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung.

Galvis Laura A LA Holik Aliaksei Z AZ Short Kieran M KM Pasquet Julie J Lun Aaron T L AT Blewitt Marnie E ME Smyth Ian M IM Ritchie Matthew E ME Asselin-Labat Marie-Liesse ML

Development (Cambridge, England) 20150319 8

Epigenetic mechanisms involved in the establishment of lung epithelial cell lineage identities during development are largely unknown. Here, we explored the role of the histone methyltransferase Ezh2 during lung lineage determination. Loss of Ezh2 in the lung epithelium leads to defective lung formation and perinatal mortality. We show that Ezh2 is crucial for airway lineage specification and alveolarization. Using optical projection tomography imaging, we found that branching morphogenesis is a ...[more]

PMID: 25790853

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