Defining the genomic occupancy of the general regulatory factor Abf1 in budding yeast

ABSTRACT: Abf1 has been defined as a general regulatory factor involved in determining chromatin structure in the yeast Saccharomyces cerevisiae. As such, it plays a major role in a range of functions including DNA replication, transcriptional activation and gene silencing, as well as DNA repair. Previous studies have identified an Abf1 DNA consensus binding sequence and Abf1 binding at up to several hundred locations throughout the genome. Depletion of Abf1 in the cell, however, results in altered nucleosome structure at many thousands of sites throughout the yeast genome, suggesting a far greater role for Abf1 in chromatin structure and dynamics. Here, we examine genome-wide Abf1 binding using ChIP-Chip to measure its chromatin occupancy. Using a novel software package, Sandcastle, we detect 3,821

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Mark Bennett 

PROVIDER: E-MTAB-3890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress