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An aging-like phenotype occurs in Tif1γ-/- hematopoietic stem cells

ABSTRACT: To determine whether an accelerated aging-like phenotype occurs in hematopoiesis of young Tif1γ-/- mice (4 months old), we purified 200,000 hematopoietic stem cells (LSK: Lineage negative, Sca1+, c-Kit+) from Tif1γ-/- mice and performed high-throughput mRNA sequencing (RNA-seq). We compared this transcriptome to physiological aging by creating two other RNAseq libraries from young (4 months old) and old (20 months old) wild type mice. RNAseq study on young Tif1γ-/- mice (4 months old), young wild type mice (4 months old) and old wild type mice (20 months old).

ORGANISM(S): Mus musculus

SUBMITTER: ronan quere

PROVIDER: E-GEOD-57396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tif1γ regulates the TGF-β1 receptor and promotes physiological aging of hematopoietic stem cells.

Quéré Ronan R Saint-Paul Laetitia L Carmignac Virginie V Martin Romain Z RZ Chrétien Marie-Lorraine ML Largeot Anne A Hammann Arlette A Pais de Barros Jean-Paul JP Bastie Jean-Noël JN Delva Laurent L

Proceedings of the National Academy of Sciences of the United States of America 20140707 29

The hematopoietic system declines with age. Myeloid-biased differentiation and increased incidence of myeloid malignancies feature aging of hematopoietic stem cells (HSCs), but the mechanisms involved remain uncertain. Here, we report that 4-mo-old mice deleted for transcription intermediary factor 1γ (Tif1γ) in HSCs developed an accelerated aging phenotype. To reinforce this result, we also show that Tif1γ is down-regulated in HSCs during aging in 20-mo-old wild-type mice. We established that T ...[more]

PMID: 25002492

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