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Molecular architecture of transcription factor hotspots in early adipogenesis

ABSTRACT: Here, we have used digital genomic footprinting to precisely define protein localization for several adipogenic transcription factors at a genome-wide level. In combination with ChIP-seq data, these analyses reveal novel molecular insight into the organization of transcription factors at hotpot regions, which provides a new framework for understanding transcription factor cooperativity on chromatin. Digital genomic footprinting and gene expression in 3T3-L1 pre-adipocytes by high throughput sequencing.

ORGANISM(S): Mus musculus

SUBMITTER: Susanne Mandrup

PROVIDER: E-GEOD-57415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular architecture of transcription factor hotspots in early adipogenesis.

Siersbæk Rasmus R Baek Songjoon S Rabiee Atefeh A Nielsen Ronni R Traynor Sofie S Clark Nicholas N Sandelin Albin A Jensen Ole N ON Sung Myong-Hee MH Hager Gordon L GL Mandrup Susanne S

Cell reports 20140522 5

Transcription factors have recently been shown to colocalize in hotspot regions of the genome, which are further clustered into super-enhancers. However, the detailed molecular organization of transcription factors at hotspot regions is poorly defined. Here, we have used digital genomic footprinting to precisely define factor localization at a genome-wide level during the early phase of 3T3-L1 adipocyte differentiation, which allows us to obtain detailed molecular insight into how transcription ...[more]

PMID: 24857666

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