Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MYC is an early response regulator of human adipogenesis in adipose stem cells

ABSTRACT: MYC is induced early in human adipose stem cells in response to a standard MDIR adipogenic cocktail. The objective of this experiment was to identify key gene networks impacted by MYC loss-of-function in a mixed donor pool of human derived adipose stem cells. Microarray analysis was used to identify up- and -down regulated genes in control vs MYC knockdown adipose stem cells in response to adipogenic stimulus Two sets of MYC siRNA oligos (pooled and s9129) were used to knockdown MYC 72 hours prior to induction of adipogenesis. Non-targeting siRNA was used as a control. Samples were induced to differentiate for 72 hours to assess early gene expression changes. Control samples not exposed to adipogenic cocktail were included as a reference. 3 independent replicates were run for each sample.

ORGANISM(S): Homo sapiens

SUBMITTER: Chad Deisenroth

PROVIDER: E-GEOD-57538 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

MYC is an early response regulator of human adipogenesis in adipose stem cells

Project description:MYC is induced early in human adipose stem cells in response to a standard MDIR adipogenic cocktail. The objective of this experiment was to identify key gene networks impacted by MYC loss-of-function in a mixed donor pool of human derived adipose stem cells. Microarray analysis was used to identify up- and -down regulated genes in control vs MYC knockdown adipose stem cells in response to adipogenic stimulus

2014-11-04 | GSE57538 | GEO

Expression data from mock- or LMO3 silenced differentiating adipose stem cells

Project description:In this study we aimed to gain further insight on the role of GCs in adipocyte differentiation. For the future drugability of candidate targets it is of utmost importance to find factors relevant to human biology. Thus, we analyzed the transcriptome of GC induced primary human adipose stem cells (hASC) to identify novel factors downstream of GC action We used microarrays to detail the global programme of gene expression following glucocorticoid treatment and identified distinct classes of up- and downregulated genes during this process. Human preadipocytes (human adipose stem cells) were obtained from lipoaspirates by enzamytic digestions, followed by several steps of centrifugations (Mikkelsen et. al Cell. 2010 Oct 1;143(1):156-69.). Following isolation, human adipose stem cells were transfected with a control siRNA (siCtrl) or two different siRNA oligos targeting the gene LMO3 (siLMO3_oligo1 or siLMO3_oligo2). 40Hrs later, the transfected human adipose stem cells were induced to differentiate into mature adipocytes with a adipogenic cocktail (FullMix) and RNA isolated at day 0 or day6 after induction of differentiation.

2013-06-12 | E-GEOD-41712 | biostudies-arrayexpress

3’RNA-seq of post-differentiation murine CD142− and CD142+ ASPCs sorted with four different anti-CD142 antibodies

Project description:To characterize CD142+ ASPCs (Aregs) after exposure to an adipogenic cocktail we performed bulk RNA-seq (using BRB-seq) of total, CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs), sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

2022-07-04 | E-MTAB-10180 | biostudies-arrayexpress

Expression data from Adipose Stem Cells (ASC) from morbidly obese and non-obese individuals

Project description:The adipose tissue is an endocrine regulator and a risk factor for atherosclerosis and cardiovascular disease when by excessive accumulation induces obesity. Although the adipose tissue is also a reservoir for stem cells (ASC) their function and “stemcellness” has been questioned. Our aim was to investigate the mechanisms by which obesity affects subcutaneous white adipose tissue (WAT) stem cells. We used microarrays to analyze differences in transcriptomic profiles between the adipose stem cells from morbidly obese and non-obese individuals. Subcutaneous white adipose tissues that were obtained during bariatric surgery (Obese) or liposuction (Lean) were donated by patients after obtaining informed consent. Three cases with BMI>40 kg/m2 (ASCmo) and three controls with BMI<25 kg/m2 (ASCn) were selected and processed extracting total RNA, processing and hybridizating on microarrays.

2013-09-17 | E-GEOD-48964 | biostudies-arrayexpress

MED31 Knockdown in Human MSCs

Project description:siRNA-mediated knockdown of MED31 and Negative siRNA control in human mesenchymal stem cells Total RNA was collected 72 hours after transfection with siRNA.

2020-09-28 | GSE116973 | GEO

Expression data fom human capillary network-derived cells before and after adipogenic differentation, and after chronic adenylate cyclase activation of differentiated cells

Project description:Progenitors in human vasculature expanded in-vitro were differentiated with adipogenic cocktail for 12 days, following which they were stimulated with forskolin for 7 days Microarrays were used to detail the program of differentiation of thermogenic human adipose cells Adipose tissue from three individuals was cultured in hydrogel under pro-angiogenic conditions. Capillary networks emerging from the explants were subcultured on plastic, grown to confluence, subjected to adipogenic differentiation and after 12 days treated with vehicle or forskolin for 7 days

2016-07-03 | E-GEOD-73385 | biostudies-arrayexpress

Expression data fom human capillary network-derived cells before and after adipogenic differentation, and after chronic adenylate cyclase activation of differentiated cells

2016-01-01 | GSE73385 | GEO

Expression data from human adipocytes after adenylate cyclase activation

2018-01-22 | GSE65190 | GEO

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA proteomics experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in a pilot experiment. Sample size: 12x12.

2022-04-07 | PXD027911 | Pride

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA secretome experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in the pilot experiment [PXDxxx (PE083)]. Sample size: 12x12.

2022-04-07 | PXD027913 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data