Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

HMeDIP-seq profiling of DNA hydroxymethylation in primary adipocytes from epididymal white adipose tissue (eWAT) of adult C57BL/6J mice

ABSTRACT: DNA hydroxymethylation plays a crucial role in the regulation of gene transcription. In this study, using hMeDIP-seq experiment, we report the mapping of DNA hydroxymethylation in adipocytes from mouse eWAT Examination of DNA hydroxymethylation pattern in adipocytes from mouse eWAT

ORGANISM(S): Mus musculus

SUBMITTER: JM-CM-)rM-CM-4me Eeckhoute

PROVIDER: E-GEOD-57582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.

Dubois-Chevalier Julie J Oger Frédérik F Dehondt Hélène H Firmin François F FF Gheeraert Céline C Staels Bart B Lefebvre Philippe P Eeckhoute Jérôme J

Nucleic acids research 20140902 17

CCCTC-binding factor (CTCF) is a ubiquitously expressed multifunctional transcription factor characterized by chromatin binding patterns often described as largely invariant. In this context, how CTCF chromatin recruitment and functionalities are used to promote cell type-specific gene expression remains poorly defined. Here, we show that, in addition to constitutively bound CTCF binding sites (CTS), the CTCF cistrome comprises a large proportion of sites showing highly dynamic binding patterns ...[more]

PMID: 25183525

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Project description:Objective: Procyanidins are polyphenolic bioactive compounds that exert beneficial effects against obesity and its related diseases. The aim of this study was to evaluate whether the supplementation with low doses of a grape seed procyanidin extract (GSPE) to dams during pre and postnatal periods has biological effects on their offspring at youth. Design: The metabolic imprinting effect of GSPE was evaluated in 30 days-old male offspring of four groups of rats that were fed either a standard diet (STD) or a high-fat diet (HFD) and supplemented with either GSPE at 25 mg per kg of body weight/day or vehicle during pregnancy and lactation. Results: A significant increase in the adiposity index and in the weight of all the white adipose tissue depots studied (retroperitoneal â??RWAT-, mesenteric â??MWAT-, epididymal â??EWAT- and inguinal â??IWAT-) was observed in offspring of dams fed with a HFD and treated with GSPE (HFT group), compared to the offspring of dams fed with the same diet and that do not received procyanidins (HF group). HFT animals also showed a higher number of cells in the EWAT, a sharply decrease of the circulating levels of monocyte chemoattractant protein-1 (MCP-1) as well as a moderate, but significant, decrease of plasma glycerol levels. The transcriptomic analysis performed in the EWAT showed 238 genes differentially expressed between HF and HFT animals, covering an entire range of processes related with the immune function and the inflammatory response (the metabolic pathway mainly reflected in the EWAT), adipose tissue remodeling and function, lipid and glucose homeostasis and metabolism of methyl groups. Conclusion: GSPE treatment to dams fed a HFD during pregnancy and lactation increases adiposity, decreases the circulating levels of MCP-1 and modulates the expression of key genes involved in the adipose tissue metabolism of their offspring. The microarray study was performed with the EWAT RNA samples of rats from the HF and the HFT groups (n=8 animals each).

Dataset Information

HMeDIP-seq profiling of DNA hydroxymethylation in primary adipocytes from epididymal white adipose tissue (eWAT) of adult C57BL/6J mice

Publications

A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.

Similar Datasets

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