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Meg3 regulated genes in pancreatic beta cells

ABSTRACT: Meg3 is a long non-coding RNA. It's target genes are unknown. The mouse pancreatic beta cell line MIN6-4N was used to assess the expression of genes upon stable Meg3 overexpression Stable cell lines were isolated that have integated pcDNA3.1 or pcDNA3.1-mMeg3.The cells were processed for RNA isolation. The level of Meg3 expression was assessed by RT-PCR. RNA preps were used for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: WeiPing Chen

PROVIDER: E-GEOD-57716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epigenetic regulation of the lncRNA MEG3 and its target c-MET in pancreatic neuroendocrine tumors.

Modali Sita D SD Parekh Vaishali I VI Kebebew Electron E Agarwal Sunita K SK

Molecular endocrinology (Baltimore, Md.) 20150107 2

Biallelic inactivation of MEN1 encoding menin in pancreatic neuroendocrine tumors (PNETs) associated with the multiple endocrine neoplasia type 1 (MEN1) syndrome is well established, but how menin loss/inactivation initiates tumorigenesis is not well understood. We show that menin activates the long noncoding RNA maternally expressed gene 3 (Meg3) by histone-H3 lysine-4 trimethylation and CpG hypomethylation at the Meg3 promoter CRE site, to allow binding of the transcription factor cAMP respons ...[more]

PMID: 25565142

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