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Gene expression in diploid and evolved tetraploid RPE-1 and BJ-1 cells

ABSTRACT: Both diploid RPE-1 and BJ-1 cells were made tetraploid by transient treatment with the cytokinesis inhibitor DCD. Proliferating tetraploids from both BJ-1 and RPE-1 were selected and isolated. The gene expression profiles of the proliferating tetraploid cells were then compared to the diploids from which they originated. Triplicate biological samples were collected and analyed.

ORGANISM(S): Homo sapiens

SUBMITTER: Neil Ganem

PROVIDER: E-GEOD-57864 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cytokinesis failure triggers hippo tumor suppressor pathway activation.

Ganem Neil J NJ Cornils Hauke H Chiu Shang-Yi SY O'Rourke Kevin P KP Arnaud Jonathan J Yimlamai Dean D Théry Manuel M Camargo Fernando D FD Pellman David D

Cell 20140801 4

Genetically unstable tetraploid cells can promote tumorigenesis. Recent estimates suggest that ∼37% of human tumors have undergone a genome-doubling event during their development. This potentially oncogenic effect of tetraploidy is countered by a p53-dependent barrier to proliferation. However, the cellular defects and corresponding signaling pathways that trigger growth suppression in tetraploid cells are not known. Here, we combine RNAi screening and in vitro evolution approaches to demonstra ...[more]

PMID: 25126788

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