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Gene expression profiling of mouse bone marrow-derived macrophages (RNA-Seq)

ABSTRACT: Bone marrow (BM) cells were obtained by flushing the long bones of 8-week old C57BL/6 mice. BM cells were then were plated in macrophage SFM medium (Life Technologies) supplemented with penicillin-streptomycin and CSF-1 (Peprotech, 100 ng/ml) and cultured for one week to allow macrophage differentiation. BMDMs were polarized by adding IL-4 to the medium (40 ng/ml, Peprotech) for 72h or left untreated. 8 samples total, 2 groups, 4 biological replicates in each group

ORGANISM(S): Mus musculus

SUBMITTER: Mark Ibberson

PROVIDER: E-GEOD-58283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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