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HepG2-MEK Inhibition with PD98059

ABSTRACT: Transcripts (mRNA) during amino acid limitation after MEK was inhibited were analyzed. HepG2 cells were pretreated with MEK inhibitor, PD98059 for 1 hour, then incubated in complete medium and a medium depleted for Histidine for 8 hours. For Inhibitor treated cells, PD98059 was included in the medium during the following 8 hours-incubation.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Kilberg

PROVIDER: E-GEOD-58869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-dependent transcriptional program controls activation of the early growth response 1 (EGR1) gene during amino acid limitation.

Shan Jixiu J Balasubramanian Mukundh N MN Donelan William W Fu Lingchen L Hayner Jaclyn J Lopez Maria-Cecilia MC Baker Henry V HV Kilberg Michael S MS

The Journal of biological chemistry 20140715 35

Amino acid (AA) limitation in mammalian cells triggers a collection of signaling cascades jointly referred to as the AA response (AAR). In human HepG2 hepatocellular carcinoma, the early growth response 1 (EGR1) gene was induced by either AA deprivation or endoplasmic reticulum stress. AAR-dependent EGR1 activation was discovered to be independent of the well characterized GCN2-ATF4 pathway and instead dependent on MEK-ERK signaling, one of the MAPK pathways. ChIP showed that constitutively boun ...[more]

PMID: 25028509

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| PRJNA253819 | ENA

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