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Microarray analysis of colonic mucosa biopsies in patients with inflammatory bowel disease

ABSTRACT: Microarrays were used to analyze the gene expression in endoscopic-derived intestinal mucosal biopsies from patients with inflammatory bowel disease (IBD) and controls Mucosal biopsies were obtained at endoscopy from the colon of 97 ulcerative colitis (UC), 8 Crohn's disease (CD) patients and 11 controls. The biopsies were taken at the most affected sites but at a distance of ulcerations. Disease activity was endoscopically assessed. Total RNA extracted from mucosal biopsies was used to analyze mRNA expression via Affymetrix Human Gene 1.0 ST arrays

ORGANISM(S): Homo sapiens

SUBMITTER: Ingrid Arijs

PROVIDER: E-GEOD-59071 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Strong Upregulation of AIM2 and IFI16 Inflammasomes in the Mucosa of Patients with Active Inflammatory Bowel Disease.

Vanhove Wiebe W Peeters Paul M PM Staelens Dominiek D Schraenen Anica A Van der Goten Jan J Cleynen Isabelle I De Schepper Sebastiaan S Van Lommel Leentje L Reynaert Niki L NL Schuit Frans F Van Assche Gert G Ferrante Marc M De Hertogh Gert G Wouters Emiel F M EF Rutgeerts Paul P Vermeire Séverine S Nys Kris K Arijs Ingrid I

Inflammatory bowel diseases 20151101 11

<h4>Background</h4>Inflammatory bowel disease (IBD) is characterized by a chronic inflammation of the gut, partly driven by defects in the innate immune system. Considering the central role of inflammasome signaling in innate immunity, we studied inflammasome components in IBD mucosa.<h4>Methods</h4>Expression of genes encoding inflammasome sensor subunits was investigated in colonic mucosal biopsies from 2 cohorts of patients with IBD and controls.<h4>Results</h4>A significant upregulation (>2- ...[more]

PMID: 26313692

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