Unknown,Transcriptomics,Genomics,Proteomics

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Polycomb Repressor Complex2 and REST-associated histone deacetylases are independent pathways toward a mature neuronal phenotype


ABSTRACT: These experiments were designed to test the hypothesis that REST and Polycomb Repressor Complex 2 function cooperatively in undifferentiated ESCs. Our results show that H3K27me3-enriched genes show no de-repression in REST-/- ESCs, while REST target genes show significant de-repression. RNA-seq was performed and analyzed using two biological replicates for each genotype, WT (N6) and REST-/- (N8) mouse embryonic stem cells.

ORGANISM(S): Mus musculus

SUBMITTER: Gail Mandel 

PROVIDER: E-GEOD-59442 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Polycomb- and REST-associated histone deacetylases are independent pathways toward a mature neuronal phenotype.

McGann James C JC   Oyer Jon A JA   Garg Saurabh S   Yao Huilan H   Liu Jun J   Feng Xin X   Liao Lujian L   Yates John R JR   Mandel Gail G  

eLife 20140924


The bivalent hypothesis posits that genes encoding developmental regulators required for early lineage decisions are poised in stem/progenitor cells by the balance between a repressor histone modification (H3K27me3), mediated by the Polycomb Repressor Complex 2 (PRC2), and an activator modification (H3K4me3). In this study, we test whether this mechanism applies equally to genes that are not required until terminal differentiation. We focus on the RE1 Silencing Transcription Factor (REST) becaus  ...[more]

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