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Gene expression signatures of human plasma cells isolated from different territories

ABSTRACT: In vivo antigen (Ag)-induced differentiation of B lymphocytes into plasma cells (PCs) takes place in extra-follicular foci and germinal centers of the secondary lymphoid organs (SLOs). Most of these SLO PCs are short-living and only relatively few PCs, characterized by secreting high-affinity antibodies (Ab), travel through the circulation and finally home in specialized survival niches of the bone marrow (BM) and, at a lesser extent, in the SLOs, where they become long-living Ab-secreting PCs. We have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between human circulating Ag-induced PCs in comparison with tonsil and BM PCs distinctively regulate genes involved in cell proliferation. Gene expressions in human plasma cells isolated from tonsil (7 samples), blood (6 samples) and bone marrow (7samples) were measured. Each sample was isolated from a different donor.

ORGANISM(S): Homo sapiens

SUBMITTER: Antonio Campos-Caro 

PROVIDER: E-GEOD-59697 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Survival of human circulating antigen-induced plasma cells is supported by plasma cell-niche cytokines and T follicular helper lymphocytes.

Ramos-Amaya Ana A   Rodríguez-Bayona Beatriz B   López-Blanco Rubén R   Andújar Eloisa E   Pérez-Alegre Mónica M   Campos-Caro Antonio A   Brieva José A JA  

Journal of immunology (Baltimore, Md. : 1950) 20141229 3

Human circulating Ag-induced plasma cells (PCs) contain a high proportion of cycling cells. This study reveals that these PCs spontaneously proliferate in culture during 72 h, as determined by BrdU-uptake detection. Transcriptome analysis indicates that, in comparison with tonsil and bone marrow (BM) PCs, these PCs distinctively upregulate genes involved in cell division. Blood PC proliferation occurs simultaneously with increasing apoptosis rates, and is associated with PC survival. In addition  ...[more]

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