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Comparative gene expression profiling of the EBV-positive NPC cell line C666.1 and normal primary tonsillar epithelial cells

ABSTRACT: In this study, we have performed comparative gene expression profiling of primary tonsil epithelial cells with the EBV-positive NPC cell line C666.1 using a human gene array. Differences in cellular gene expression was compared using RNA isolated from C666-1 cells and primary tonsil epithelial cells grown in serum-free keratinocyte growth medium (performed in triplicate). RNA isolated from each sample was processed according to the Genechip WT sense target labelling protocol. 1μg of RNA was hybridised to human gene 1.0 ST array chips.

ORGANISM(S): Homo sapiens

SUBMITTER: Wenbin Wei

PROVIDER: E-GEOD-39826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epstein-Barr virus induction of the Hedgehog signalling pathway imposes a stem cell phenotype on human epithelial cells.

Port Rebecca J RJ Pinheiro-Maia Sonia S Hu Chunfang C Arrand John R JR Wei Wenbin W Young Lawrence S LS Dawson Christopher W CW

The Journal of pathology 20131101 3

Nasopharyngeal carcinoma (NPC) is a cancer common in southern China and South East Asia that is causally linked to Epstein-Barr virus (EBV) infection. Here, we demonstrate that NPC displays frequent dysregulation of the Hedgehog (HH) pathway, a pathway implicated in the maintenance of stem cells, but whose aberrant activation in adult tissues can lead to cancer. Using authentic EBV-positive carcinoma-derived cell lines and nasopharyngeal epithelial cell lines latently infected with EBV as models ...[more]

PMID: 23934731

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