Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from adult mouse cortex harvested at two different zeitgeber (ZT) timepoints of the 24h cycle

ABSTRACT: Gene expression in forebrain structures change during day and night depending on circadian and rest-activity cycles. Clock genes have been shown to be involved in the control of circadian and sleep-wake control. In this study, we aimed at studying the consequences of loss of SHARP1 (DEC2/BHLHE40) and SHARP2 (DEC1/BHLHE41) on cortical gene expression at rest (ZT4) and activity (ZT16) phases. We harvested cortex tissue from individual mice from WT and SHARP1/2 double null mutant mice at ZT4 and ZT16, prepared total RNA and subjected the corresponding samples (n=2 biological replicates per timepoint and genotype) to Affymetrix genechip analysis to assay the changes of gene expression.

ORGANISM(S): Mus musculus

SUBMITTER: Moritz Rossner

PROVIDER: E-GEOD-59941 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mice lacking the circadian modulators SHARP1 and SHARP2 display altered sleep and mixed state endophenotypes of psychiatric disorders.

Baier Paul C PC Brzózka Magdalena M MM Shahmoradi Ali A Reinecke Lisa L Kroos Christina C Wichert Sven P SP Oster Henrik H Wehr Michael C MC Taneja Reshma R Hirrlinger Johannes J Rossner Moritz J MJ

PloS one 20141023 10

Increasing evidence suggests that clock genes may be implicated in a spectrum of psychiatric diseases, including sleep and mood related disorders as well as schizophrenia. The bHLH transcription factors SHARP1/DEC2/BHLHE41 and SHARP2/DEC1/BHLHE40 are modulators of the circadian system and SHARP1/DEC2/BHLHE40 has been shown to regulate homeostatic sleep drive in humans. In this study, we characterized Sharp1 and Sharp2 double mutant mice (S1/2-/-) using online EEG recordings in living animals, be ...[more]

PMID: 25340473

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Project description:Using high throughput sequencing of Drosophila head RNA, a small set of miRNAs that undergo robust circadian oscillations in levels were discovered. We concentrated on a cluster of six miRNAs, mir-959-964, all of which peak at about ZT12 or lights-off. The data indicate that the cluster pri-miRNA is transcribed under bona fide circadian transcriptional control and that all 6 mature miRNAs have short half-lives, a requirement for oscillating. Manipulation of food intake dramatically affects the levels and timing of cluster miRNA transcription with no more than minor effects on the core circadian oscillator. This indicates that the central clock regulates feeding, which in turn regulates proper levels and cycling of the cluster miRNAs. Viable Gal4 knock-in as well as cluster knock-out and over-expression strains were used to localize cluster miRNA expression as well as explore their functions. The adult head fat body is a major site of expression, and feeding behavior, innate immunity, metabolism, and perhaps stress responses are under cluster miRNA regulation. The feeding behavior results indicate that there is a feedback circuit between feeding time and cluster miRNA function as well as a surprising role of post-transcriptional regulation in these behaviors and physiology. To address possible functions of the cluster miRNAs, the knock-out strain (mir959-952-KO) as well as a cluster over-expression strain (UAS-cluster, tim-gal4) was assayed for mRNA changes relative to their WT counterparts on Affymetrix expression arrays. The strategy was based on the observation that miRNAs often cause a decrease in the steady-state levels of their target mRNAs (Guo et al., 2010; Lim et al., 2005). Because of the circadian regulation and the possibility of non-fat body expression, the over-expression strain was generated with the broad circadian driver tim-gal4 rather than a fat body driver. To accommodate the possibility that important mRNA changes only appear at certain circadian times, RNA was assayed from heads collected at two different times, ZT4 and ZT16. Analyzed expression data was assayed from total RNA from Drosophila heads in two sample groups consisting of four samples (with eight samples in total): knock-out strain at ZT4 and ZT16, and its respective wildtype control at ZT4 and ZT16; cluster over-expression strain at ZT4 and ZT16 and its respective wildtype control at ZT4 and ZT16.

Dataset Information

Expression data from adult mouse cortex harvested at two different zeitgeber (ZT) timepoints of the 24h cycle

Publications

Mice lacking the circadian modulators SHARP1 and SHARP2 display altered sleep and mixed state endophenotypes of psychiatric disorders.

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