Proteomics

Dataset Information

0

Sleep-wake cycles drive daily dynamics of synaptic phosphorylation

ABSTRACT: The circadian clock drives daily changes of physiology, including sleep-wake cycles, by regulating transcription, protein abundance and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24h, accurately quantifying almost 8,000 phosphopeptides. Remarkably, half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function via phosphorylation, including synaptic transmission, cytoskeleton reorganization and excitatory/inhibitory balance. Remarkably, sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Mario Oroshi  

LAB HEAD: Maria S Robles

PROVIDER: PXD010697 | Pride | 2019-10-14

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20170123_Qep6_ChRo_SA_collab_SYN_CT_phospho_1.raw Raw
20170123_Qep6_ChRo_SA_collab_SYN_CT_phospho_10.raw Raw
20170123_Qep6_ChRo_SA_collab_SYN_CT_phospho_11.raw Raw
20170123_Qep6_ChRo_SA_collab_SYN_CT_phospho_12.raw Raw
20170123_Qep6_ChRo_SA_collab_SYN_CT_phospho_13.raw Raw
Items per page:
1 - 5 of 98
altmetric image

Publications

The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep.

Noya Sara B SB   Colameo David D   Brüning Franziska F   Spinnler Andrea A   Mircsof Dennis D   Opitz Lennart L   Mann Matthias M   Tyagarajan Shiva K SK   Robles Maria S MS   Brown Steven A SA  

Science (New York, N.Y.) 20191001 6462

Neurons have adapted mechanisms to traffic RNA and protein into distant dendritic and axonal arbors. Taking a biochemical approach, we reveal that forebrain synaptic transcript accumulation shows overwhelmingly daily rhythms, with two-thirds of synaptic transcripts showing time-of-day-dependent abundance independent of oscillations in the soma. These transcripts formed two sharp temporal and functional clusters, with transcripts preceding dawn related to metabolism and translation and those anti  ...[more]

PMID: 31601739

Publication: 1/2

Similar Datasets

Transcriptomics Expression data from adult mouse cortex harvested at two different zeitgeber (ZT) timepoints of the 24h cycle
2014-11-21 | GSE59941 | GEO
Transcriptomics Expression data from adult mouse cortex harvested at two different zeitgeber (ZT) timepoints of the 24h cycle
2014-11-21 | E-GEOD-59941 | biostudies-arrayexpress
Transcriptomics Circadian and Sleep regulation of synaptic RNA
2019-02-28 | E-MTAB-7347 | biostudies-arrayexpress
Transcriptomics Single-cell transcriptomics reveals glial cells integrate homeostatic and circadian processes to drive sleep-wake cycle
2023-11-29 | GSE221239 | GEO
Transcriptomics Coordination of hunger and sleep through circadian regulation of transcription in energy-sensing neurons
2019-03-04 | GSE124693 | GEO
Transcriptomics Circadian VIPergic neurons of the suprachiasmatic nuclei sculpt the sleep-wake cycle
2021-01-21 | GSE154038 | GEO
Proteomics Single-cell transcriptomics and cell-specific proteomics reveals new molecular regulators of sleep
2022-10-13 | PXD018334 | Pride
Proteomics Homer1a drives Homeostatic Scaling-down of Excitatory Synapses During Sleep
2017-02-13 | PXD004537 | Pride
Proteomics Circadian Proteomics Landscape of Mouse Livers Organized by Time-restricted Feeding: Phosphorylation
2023-08-22 | PXD038336 | Pride
Proteomics Proteomics of human peripheral blood mononuclear cells under normal circadian cycle and under a simulated night shift
2023-10-05 | MSV000093054 | MassIVE