Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human 27 non-labored normal preterm and 9 non-labored normal term basal plate samples to investigate gene expression at the human maternal-fetal interface

ABSTRACT: Microarray analysis to globally assess gene expression at the maternal-fetal interface Experiment Overall Design: 27 non-labored normal preterm and 9 non-labored normal term basal plate samples were analyzed on Affymetrix U133 chips using established protocols. The gene expression profile of preterm was compared to term.

ORGANISM(S): Homo sapiens

SUBMITTER: Susan Fisher

PROVIDER: E-GEOD-5999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression profiling of the human maternal-fetal interface reveals dramatic changes between midgestation and term.

Winn Virginia D VD Haimov-Kochman Ronit R Paquet Agnes C AC Yang Y Jean YJ Madhusudhan M S MS Gormley Matthew M Feng Kui-Tzu V KT Bernlohr David A DA McDonagh Susan S Pereira Lenore L Sali Andrej A Fisher Susan J SJ

Endocrinology 20061214 3

Human placentation entails the remarkable integration of fetal and maternal cells into a single functional unit. In the basal plate region (the maternal-fetal interface) of the placenta, fetal cytotrophoblasts from the placenta invade the uterus and remodel the resident vasculature and avoid maternal immune rejection. Knowing the molecular bases for these unique cell-cell interactions is important for understanding how this specialized region functions during normal pregnancy with implications f ...[more]

PMID: 17170095

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Project description:Preterm birth is a main determinant of neonatal mortality and morbidity and a major contributor to the overall mortality and burden of disease. However, precise phenotyping of the preterm birth is hampered by the imprecise definition of the clinical phenotype and complexity of the molecular phenotype due to multiple pregnancy tissue types and molecular processes that may contribute to the preterm birth. The studyâ??s aim was to comprehensively evaluate the mRNA transcriptome that characterizes preterm and term labor using precisely phenotyped samples. Expression profiles of 73 genes and non-coding RNA sequences uniquely identified the four groups of patients: delivering preterm with (PL) and without labor (PNL), term with (TL) and without labor (TNL). The largest differences in gene expression among the four groups occurred in decidua, chorion and amnion. The gene expression profiles showed suppression of chemokines expression in TNL, withdrawal of this suppression in TL, activation of multiple pathways of inflammation in PL, and an immune rejection profile in PNL. The genes constituting expression signatures showed over-representation of three putative regulatory elements in their 5â?? and 3â??UTR regions. The results suggest that pregnancy is maintained by downregulation of chemokines at the maternal-fetal interface. Withdrawal of this downregulation results in the term birth and its overriding by the activation of multiple pathways of the immune system in the preterm birth. Complications of the pregnancy associated with impairment of placental function, which necessitated premature delivery of the fetus in the absence of labor, show gene expression patterns associated with immune rejection. 183 total RNA samples from 8 tissue types collected from 35 women grouped into six categories of pregnancy outcome. One microarray replicate per sample. Other Contributors: Radek Bukowski, Sam Parry and the NICHD Genomic and Proteomic Network for Preterm Birth Research

Dataset Information

Transcription profiling of human 27 non-labored normal preterm and 9 non-labored normal term basal plate samples to investigate gene expression at the human maternal-fetal interface

Publications

Gene expression profiling of the human maternal-fetal interface reveals dramatic changes between midgestation and term.

Similar Datasets

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