Genome-wide miRNA turnover profile after transcription inhibition in HDMYZ cells with biological triplicate
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ABSTRACT: HDMYZ cells were treated with 4ug/ml ActD for 0, 1, 4 and 12 hours. Small RNAs of 15-40 bases were gel-purified from 5 ug total RNA, and subjected to multiplex Illumina small RNA library preparation. Small RNA libraries were sequenced on a HiSeq2000 (Illumina). Measure miRNA expression level at 0, 1, 4, 12 hours post ActinomycinD treatment to examine miRNA turnover pattern.
ORGANISM(S): Homo sapiens
SUBMITTER: Mei Zhong
PROVIDER: E-GEOD-60036 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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