Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profile of human lymphatic endothelial cells under static or oscillatory shear stress conditions in the presence or absence of FOXC2

ABSTRACT: Lymphatic valves are specialized units regularly distributed along collecting vessels that allow unidirectional forward propulsion of the lymph, and its efficient transport from tissues to the bloodstream. Lymphatic endothelial cells that cover lymphatic valve sinuses are subjected to complex flow patterns, due to recirculation of the lymph during the collecting vessel pumping cycle. They also express high levels of FOXC2 transcription factor. We used microarrays to study the transcriptional networks controlled by FOXC2 in human lymphatic endothelial cells subjected to oscillatory shear stress or cultured under static conditions. Human lymphatic endothelial cells were transfected with control or FOXC2 siRNAs and subjected to 24-hour oscillatory shear stress (1 dyn/cm2; 1/4 Hz) or kept under static conditions as a control. RNA were amplified and hybridized on Affymetrix Human Gene 1.0 ST Arrays. The experiment was run twice independently, using each time a different siRNA to knockdown FOXC2, as previously described (Sabine et al, 2012, Dev Cell).

ORGANISM(S): Homo sapiens

SUBMITTER: Nadine Zangger

PROVIDER: E-GEOD-60152 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature.

Sabine Amélie A Bovay Esther E Demir Cansaran Saygili CS Kimura Wataru W Jaquet Muriel M Agalarov Yan Y Zangger Nadine N Scallan Joshua P JP Graber Werner W Gulpinar Elgin E Kwak Brenda R BR Mäkinen Taija T Martinez-Corral Inés I Ortega Sagrario S Delorenzi Mauro M Kiefer Friedemann F Davis Michael J MJ Djonov Valentin V Miura Naoyuki N Petrova Tatiana V TV

The Journal of clinical investigation 20150921 10

Biomechanical forces, such as fluid shear stress, govern multiple aspects of endothelial cell biology. In blood vessels, disturbed flow is associated with vascular diseases, such as atherosclerosis, and promotes endothelial cell proliferation and apoptosis. Here, we identified an important role for disturbed flow in lymphatic vessels, in which it cooperates with the transcription factor FOXC2 to ensure lifelong stability of the lymphatic vasculature. In cultured lymphatic endothelial cells, FOXC ...[more]

PMID: 26389677

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Dataset Information

Expression profile of human lymphatic endothelial cells under static or oscillatory shear stress conditions in the presence or absence of FOXC2

Publications

FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature.

Similar Datasets

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