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Effect of knock down of LASP-1 on luminal breast cancer cells (MCF7)

ABSTRACT: Nuclear LASP-1 has a direct correlation with the overall survival of breast cancer patients. Gene expression analysis of MCF7 human breast cancer cells cultured in 3D-Matrigel was performed. Up regulation of cell junction proteins, extracellular matrix proteins and down regulation of MMP 9 and 2 were observed. This corroborates well with the involvement of LASP-1 in cell migration and chemotaxis. Non-silencing (control) and LASP-1 knock down MCF7 cells were cultured on 3D-Matrigel, total RNA was extracted and anlyzed - 4 biological replicates each. The first biological replicate was done as a pilot. Biological replicates 2-4 were run as a second set after pilot.

ORGANISM(S): Homo sapiens

SUBMITTER: Dayanidhi Raman

PROVIDER: E-GEOD-60322 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex.

Duvall-Noelle N N Karwandyar A A Richmond A A Raman D D

Oncogene 20150518 9

Nuclear LASP-1 (LIM and SH3 protein-1) has a direct correlation with overall survival of breast cancer patients. In this study, immunohistochemical analysis of a human breast TMA showed that LASP-1 is absent in normal human breast epithelium but the expression increases with malignancy and is highly nuclear in aggressive breast cancer. We investigated whether the chemokines and growth factors present in the tumor microenvironment could trigger nuclear translocation of LASP-1.Treatment of human b ...[more]

PMID: 25982273

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