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MicroRNA analysis on epithelial D492 cells and its mesenchymal derivative D492M cells

ABSTRACT: miRNA analysis of breast epithelial cell line with stem cell properties before and after undergoing endothelial induced epithelial to mesenchymal transition (EMT). miRNA expression anlysis was done on breast epithelial cells before and after endothelial induced EMT. D492 is a breast epithelial cell line with stem cell properties that undergoes EMT in 3D rBM coculture with endothelial cells. Total RNA was isolated from D492 and D492M (a mesenchymal derivative) at 50% and 90% confluency in monolayer.

ORGANISM(S): Homo sapiens

SUBMITTER: Markus Ringnér

PROVIDER: E-GEOD-60524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

Hilmarsdóttir Bylgja B Briem Eirikur E Sigurdsson Valgardur V Franzdóttir Sigrídur Rut SR Ringnér Markus M Arason Ari Jon AJ Bergthorsson Jon Thor JT Magnusson Magnus Karl MK Gudjonsson Thorarinn T

Developmental biology 20150509 2

The epithelial compartment of the breast contains two lineages, the luminal- and the myoepithelial cells. D492 is a breast epithelial cell line with stem cell properties that forms branching epithelial structures in 3D culture with both luminal- and myoepithelial differentiation. We have recently shown that D492 undergo epithelial to mesenchymal transition (EMT) when co-cultured with endothelial cells. This 3D co-culture model allows critical analysis of breast epithelial lineage development and ...[more]

PMID: 25967125

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MicroRNA analysis on epithelial D492 cells and its mesenchymal derivative D492M cells

Project description:miRNA analysis of breast epithelial cell line with stem cell properties before and after undergoing endothelial induced epithelial to mesenchymal transition (EMT).

2015-07-02 | GSE60524 | GEO

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2011-05-27 | E-GEOD-27883 | biostudies-arrayexpress

TGF-b1 stimulates lymphatic endothelial cells to produce IL-7 and IL-15, which act as chemotactic factors for breast cancer cells with EMT properties

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2012-07-13 | E-GEOD-39359 | biostudies-arrayexpress

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2013-11-13 | E-GEOD-52262 | biostudies-arrayexpress

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