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Dormancy within Staphylococcus epidermidis biofilms: a transcriptomic analysis by RNA-seq

ABSTRACT: We sequenced mRNA from three independent biological replicates of Staphylococcus epidermidis biofilms with different proportion of dormant cells. Whole trancriptome analysis of Staphylococcus epidermidis biofilms with prevented and induced dormancy.

ORGANISM(S): Staphylococcus epidermidis

SUBMITTER: Virginia Carvalhais

PROVIDER: E-GEOD-60834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dormancy within Staphylococcus epidermidis biofilms: a transcriptomic analysis by RNA-seq.

Carvalhais Virginia V França Angela A Cerca Filipe F Vitorino Rui R Pier Gerald B GB Vilanova Manuel M Cerca Nuno N

Applied microbiology and biotechnology 20140207 6

The proportion of dormant bacteria within Staphylococcus epidermidis biofilms may determine its inflammatory profile. Previously, we have shown that S. epidermidis biofilms with higher proportions of dormant bacteria have reduced activation of murine macrophages. RNA-sequencing was used to identify the major transcriptomic differences between S. epidermidis biofilms with different proportions of dormant bacteria. To accomplish this goal, we used an in vitro model where magnesium allowed modulati ...[more]

PMID: 24504458

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Dormancy within Staphylococcus epidermidis biofilms: a transcriptomic analysis by RNA-seq

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