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Molecular control of myeloid suppressors by death pathways

ABSTRACT: The goal of this study was to evaluate the mechanisms distinguishing the different forms of myeloid suppressors in cancer and inflammation, and their indivdual roles in T cell suppression We used microarrays to profile the expression profile of myeloid suppressor cells in mouse and human samples. The starting point is the attached arrays which use different CD11+ or CD14+ selections to crudely separate the myeloid suppressors from other immune cells. The overall study is an initial step towards much higher resolution genetics studies to determine the functional prathways used by myeloid suppressors to inhibit T-cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Geoffrey Neale

PROVIDER: E-GEOD-61479 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Myeloid-derived suppressor activity is mediated by monocytic lineages maintained by continuous inhibition of extrinsic and intrinsic death pathways.

Haverkamp Jessica M JM Smith Amber M AM Weinlich Ricardo R Dillon Christopher P CP Qualls Joseph E JE Neale Geoffrey G Koss Brian B Kim Young Y Bronte Vincenzo V Herold Marco J MJ Green Douglas R DR Opferman Joseph T JT Murray Peter J PJ

Immunity 20141211 6

Nonresolving inflammation expands a heterogeneous population of myeloid suppressor cells capable of inhibiting T cell function. This heterogeneity has confounded the functional dissection of individual myeloid subpopulations and presents an obstacle for antitumor immunity and immunotherapy. Using genetic manipulation of cell death pathways, we found the monocytic suppressor-cell subset, but not the granulocytic subset, requires continuous c-FLIP expression to prevent caspase-8-dependent, RIPK3-i ...[more]

PMID: 25500368

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