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Sperm miRNA expression differences between bulls consuming toxic and non-toxic tall fescue seed

ABSTRACT: The objectives of our study were to identify microRNA (miRNA) present in bovine sperm and to evaluate the effects of fescue toxicosis on sperm miRNA expression. Angus bulls were assigned to treatments of either toxic or non-toxic fescue seed diets. Semen was collected and subjected to microRNA (miRNA) isolation after 126 days. Three bull's sperm miRNA samples from each treatment group were chosen and pooled for deep sequencing. Sequencing results were used to create a custom microarray for miRNA comparison between groups. LC Sciences was used as a service provider for the sequencing and custom microarray.

ORGANISM(S): Bos taurus

SUBMITTER: Scott Pratt 

PROVIDER: E-GEOD-61745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The bull sperm microRNAome and the effect of fescue toxicosis on sperm microRNA expression.

Stowe Heather M HM   Calcatera Samantha M SM   Dimmick Marcy A MA   Andrae John G JG   Duckett Susan K SK   Pratt Scott L SL  

PloS one 20141202 12

Tall fescue [Schedonorus phoenix (Scop.) Holub] accounts for nearly 16 million hectares of pasture in the Southeastern and Mid-Atlantic U.S. due to its heat, drought, and pest resistance, conferred to the plant by its symbiotic relationship with the endophyte Neotyphodium coenophialum. The endophyte produces ergot alkaloids that have negative effects on the growth and reproduction of animals, resulting in the syndrome known as fescue toxicosis. The objectives of our study were to identify microR  ...[more]

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