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Deep sequencing of microRNA present in ejaculated bull spermatozoa

ABSTRACT: The objectives of our study were to identify microRNA (miRNA) present in bovine sperm. Angus bulls (n=6) were assigned to treatments of either toxic or non-toxic fescue seed diets. Semen was collected and subjected to RNA isolation, enriched for miRNA. Three samples from each treatment group were chosen based on RNA quality and pooled for deep sequencing.

ORGANISM(S): Bos taurus

SUBMITTER: Scott Pratt

PROVIDER: E-GEOD-61746 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The bull sperm microRNAome and the effect of fescue toxicosis on sperm microRNA expression.

Stowe Heather M HM Calcatera Samantha M SM Dimmick Marcy A MA Andrae John G JG Duckett Susan K SK Pratt Scott L SL

PloS one 20141202 12

Tall fescue [Schedonorus phoenix (Scop.) Holub] accounts for nearly 16 million hectares of pasture in the Southeastern and Mid-Atlantic U.S. due to its heat, drought, and pest resistance, conferred to the plant by its symbiotic relationship with the endophyte Neotyphodium coenophialum. The endophyte produces ergot alkaloids that have negative effects on the growth and reproduction of animals, resulting in the syndrome known as fescue toxicosis. The objectives of our study were to identify microR ...[more]

PMID: 25462855

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Dynamics of microRNAs in bull spermatozoa.

Project description:MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions.To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets.We demonstrated that an abundance of microRNAs were present in bovine spermatozoa, however, only seven were differentially expressed; hsa-aga-3155, -8197, -6727, -11796, -14189, -6125, -13659. The abundance of miRNAs in the spermatozoa and the differential expression in sperm from high vs. low fertility bulls suggests that the miRNAs possibly play important functions in the regulating mechanisms of bovine spermatozoa.Identification of specific microRNAs expressed in spermatozoa of bulls with different fertility phenotypes will help better understand mammalian gametogenesis and early development.

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