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Transcription profiling of Drosophila wild type and sca-gcm mutant neuroectodermal cells, selected by magnetic cell separation, to understand Drosophila gliogenesis during early embryogenesis

ABSTRACT: We report here the application of magnetic cell separation in microarray experiment to understand Drosophila gliogenesis during early embryogenesis. At stage 11, neuroectodermal cells were labeled by a fusion transmembrane protein, mCD8-GFP, in the wild-type embryos as well as in the embryos where glide/gcm was overexpressed. Following the cell dissociation, neuroectodermal cells were purified by using the commercial available anti-mCD8 microbeads. Microarray experiments based on prepared neuroectodermal cells from wild-type embryos verse glide/gcm overexpressed embryos at stage 11 have revealed 76 gene differentially regulated by overexpression of glide/gcm.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Haiqiong Montalta-He

PROVIDER: E-GEOD-618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila gliogenesis during early embryogenesis

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