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Gene Expression Profiling of Glycine max Cotyledons and Trifoliate Leaves to Determine Genes Associated with Senescence

ABSTRACT: We use RNA sequencing technology in a time course study to measure transcript abundance from three developmental stages in cotyledons and five stages in the trifoliate leaf of Glycine max to identify genes with distinct temporal patterns of expression during leaf or cotyledon development. We also examine the differences between these two photosynthetic tissues. Time course Expression analysis of Cotyledon Development and Leaf Development using RNAseq on distinct time points. 3 stages of the cotyledon were sequenced with 3 biological replicates in each stage. Five stages of the leaf, determined by physical appearance and plant developmental stage, were collected from the time of soil emergence to the onset of senescence and were sequenced with 3 biological replicates for each stage.

ORGANISM(S): Glycine max

SUBMITTER: Karen Hudson

PROVIDER: E-GEOD-61857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons.

Brown Anne V AV Hudson Karen A KA

BMC plant biology 20150703

<h4>Background</h4>Immediately following germination, the developing soybean seedling relies on the nutrient reserves stored in the cotyledons to sustain heterotrophic growth. During the seed filling period, developing seeds rely on the transport of nutrients from the trifoliate leaves. In soybean, both cotyledons and leaves develop the capacity for photosynthesis, and subsequently senesce and abscise once their function has ended. Before this occurs, the nutrients they contain are mobilized and ...[more]

PMID: 26149852

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