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S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells

ABSTRACT: Genome-wide DNA methylation profiling of human PC3 invasive prostate cancer cell line treated with vehicle control (SAH, S-adenosylhomocysteine) and with SAM (S-adenosylmethionine) as well as of untreated human LNCaP non-invasive prostate cancer cell line. The Illumina Infinium 450k Human DNA Methylation BeadChip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs in human cell lines exposed to described treatments. Samples included biological triplicate of PC3 control (SAH treated), biological triplicate of PC3 treated with SAM, and biological duplicate of LNCaP untreated. Bisulfite-converted DNA from the 8 samples were hybridised to the Illumina Infinium 450k Human Methylation BeadChip v1.2.

ORGANISM(S): Homo sapiens

SUBMITTER: Barbara Stefanska 

PROVIDER: E-GEOD-62053 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pharmacological methyl group donors block skeletal metastasis in vitro and in vivo.

Shukeir Nicholas N   Stefanska Barbara B   Parashar Surabhi S   Chik Flora F   Arakelian Ani A   Szyf Moshe M   Rabbani Shafaat A SA  

British journal of pharmacology 20150327 11

<h4>Background and purpose</h4>DNA hypomethylation was previously implicated in metastasis. In the present study, we examined whether methyl supplementation with the universal methyl donor S-adenosylmethionine (SAM) inhibits prostate cancer associated skeletal metastasis.<h4>Experimental approach</h4>Highly invasive human prostate cancer cells PC-3 and DU-145 were treated with vehicle alone, S-adenosylhomocysteine (SAH) or SAM and their effects on tumour cell proliferation, invasion, migration a  ...[more]

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