Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression in M. tuberculosis and M. africanum infected tuberculosis patients prior to and following treatment

ABSTRACT: Differential gene expression in M. tuberculosis and M. africanum patients under TB treatment Gene expression analysis of whole blood RNA from tuberculosis patients prior to and following treatment

ORGANISM(S): Homo sapiens

SUBMITTER: Jeroen Maertzdorf

PROVIDER: E-GEOD-62147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Differential transcriptomic and metabolic profiles of M. africanum- and M. tuberculosis-infected patients after, but not before, drug treatment.

Tientcheu L D LD Maertzdorf J J Weiner J J Adetifa I M IM Mollenkopf H-J HJ Sutherland J S JS Donkor S S Kampmann B B Kaufmann S H E SH Dockrell H M HM Ota M O MO

Genes and immunity 20150604 5

The epidemiology of Mycobacterium tuberculosis (Mtb) and M. africanum (Maf) suggests differences in their virulence, but the host immune profile to better understand the pathogenesis of tuberculosis (TB) have not been studied. We compared the transcriptomic and metabolic profiles between Mtb- and Maf-infected TB cases to identify host biomarkers associated with lineages-specific pathogenesis and response to anti-TB chemotherapy. Venous blood samples from Mtb- and Maf-infected patients obtained b ...[more]

PMID: 26043170

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Project description:Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact on human and animal health worldwide. Mycobacterial life cycle is complex and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural reservoir hosts for MTBC and a model for mycobacterial infection and tuberculosis (TB). In the wild boar TB model, mycobacterial infection affects the expression of innate and adaptive immune response genes in mandibular lymph nodes and oropharyngeal tonsils and biomarkers have been proposed as correlates with resistance to natural infection. However, the mechanisms used by mycobacteria to manipulate host immune response are not fully characterized. Our hypothesis is that the immune system proteins under-represented in infected animals when compared to uninfected controls are used by mycobacteria to guarantee pathogen infection and transmission. To address this hypothesis, a comparative proteomics approach was used to compare host response between uninfected (TB-) and M. bovis-infected young (TB+) and adult animals with different infection status [TB lesions localized in the head (TB+) or affecting multiple organs (TB++)]. The results identified host immune system proteins that play an important role in host response to mycobacteria. Calcium binding protein A9, Heme peroxidase, Lactotransferrin, Cathelicidin and Peptidoglycan-recognition protein were under-represented in TB+ animals when compared to uninfected TB- controls but protein levels increased as infection progressed in TB++ animals when compared to TB- and/or TB+ adult wild boar. MHCI was the only protein over-represented in TB+ adult wild boar when compared to uninfected TB- controls. The results reported here suggested that M. bovis manipulates host immune response by reducing the production of immune system proteins. However, as infection progresses, wild boar immune response recover to limit pathogen multiplication and promote survival that also facilitates pathogen transmission.

Dataset Information

Gene expression in M. tuberculosis and M. africanum infected tuberculosis patients prior to and following treatment

Publications

Differential transcriptomic and metabolic profiles of M. africanum- and M. tuberculosis-infected patients after, but not before, drug treatment.

Similar Datasets

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