Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome Analysis of KSHV during de novo primary infection of human B cells (PBMCs)

ABSTRACT: The objective of this study was to identify the viral transcripts packaged into the virion particles and the transcription profiles of the viral genome during early infection, till the virus establishes latent infection in human PBMCs Samples from each time points were analyzed and the experiments were done in duplicate.

ORGANISM(S): Human herpesvirus 8

SUBMITTER: Subhash Verma

PROVIDER: E-GEOD-62341 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome Analysis of KSHV during de novo primary infection of human CD14+ cells

Project description:The objective of this study was to identify the viral transcripts packaged into the virion particles and the transcription profiles of the viral genome during early infection, till the virus establishes latent infection in B-cells as well as in endothelial cells Samples from each time points were analyzed and the experiments were done in duplicate.

2014-10-16 | E-GEOD-62340 | biostudies-arrayexpress

Transcriptome Analysis of KSHV during de novo primary infection of endothelial (TIVE) cells

Project description:The objective of this study was to identify the viral transcripts packaged into the virion particles and the transcription profiles of the viral genome during early infection, till the virus establishes latent infection in endothelial (TIVE) cells Samples from each time points were analyzed and the experiments were done in duplicate.

2014-10-16 | E-GEOD-62342 | biostudies-arrayexpress

Transcriptome Analysis of KSHV during de novo primary infection of human B-and endothelial-cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-10-16 | E-GEOD-62344 | biostudies-arrayexpress

Transcriptome Analysis of BAC36 and ORF59 deleted BAC36 during de novo primary infection of human B cells (PBMCs)

Project description:The objective of this study was to identify the transcription profiles of wild type KSHV and ORF59 deleted KSHV (Kaposi's sarcoma-associated herpesvirus) genome during early infection, till the virus establishes latent infection in human PBMCs Samples from each time points were analyzed and the experiments were done in duplicate.

2014-10-16 | E-GEOD-62339 | biostudies-arrayexpress

Transcriptome Analysis of KSHV virions produced from BCBL1 and BAC36 in 293L cells

Project description:The objective of this study was to identify the viral transcripts packaged into the virion particles produced from BCBL1 cells as well as virions from 293L cells containing BAC36 BACs RNA from virions were extracted and analyzed by RNA sequencing

2014-10-16 | E-GEOD-62343 | biostudies-arrayexpress

Transcriptome Analysis of KSHV during de novo primary infection of endothelial (TIVE) cells

2014-10-16 | GSE62342 | GEO

Transcriptome Analysis of KSHV during de novo primary infection of human B cells (PBMCs)

2014-10-16 | GSE62341 | GEO

Transcriptome Analysis of KSHV virions produced from BCBL1 and BAC36 in 293L cells

2014-10-16 | GSE62343 | GEO

Ago HITS-CLIP in KSHV-infected primary effusion lymphoma (PEL) cell lines BCBL-1 and BC-3

Project description:We performed Ago HITS-CLIP to identify targets of viral and human miRNAs in latently KSHV-infected PEL cells Ago HITS-CLIP was performed in two latently infected PEL cell lines, BCBL-1 and BC-3; Argonaute-immunoprecipitation of UV cross-linked Ago-miRNA-mRNA complexes, followed by RNA isolation, library construction, and high-throughput sequencing (Illumina GAxII); we performed 3 biological replicates for each cell line, two technical (sequencing) replicates of BCBL-1 biological replicate 1

2012-10-05 | E-GEOD-41357 | biostudies-arrayexpress

An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus

Project description:The virion proteins of Kaposi Sarcoma Associated Herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined detected 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via Ultra-High Resolution Qq-Time-Of-Flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins.

2020-12-10 | PXD022626 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data