Proteomics

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An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus


ABSTRACT: The virion proteins of Kaposi Sarcoma Associated Herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined detected 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via Ultra-High Resolution Qq-Time-Of-Flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins.

INSTRUMENT(S): impact II

ORGANISM(S): Human Herpesvirus 8 Strain Gk18

DISEASE(S): Kaposi's Sarcoma

SUBMITTER: basir syed  

LAB HEAD: Jennifer E. Totonchy

PROVIDER: PXD022626 | Pride | 2020-12-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BCM20KSHV_RA2_1_340.mgf Mgf
BCMKSHV_RA2_1_341.mgf Mgf
HHV8NP69C_TM_RA8_1_347.mgf Mgf
HHV8NP69M_TM_RA1_1_339.mgf Mgf
HHV8NP6BC_TM_RA6_1_345.mgf Mgf
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Publications

An Update of the Virion Proteome of Kaposi Sarcoma-Associated Herpesvirus.

Nabiee Ramina R   Syed Basir B   Ramirez Castano Jesus J   Lalani Rukhsana R   Totonchy Jennifer E JE  

Viruses 20201202 12


The virion proteins of Kaposi sarcoma-associated herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined the detection of 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the prot  ...[more]

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