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Transcription and Imprinting Dynamics in Developing Postnatal Male Germline Stem Cells

ABSTRACT: Paternal imprinting initiates in primordial germ cells (PGCs), and is considered largely completed at birth. The resulting postnatal spermatogonial stem cells (SSCs) thenself-renew and proliferate to populate the testicular niche, with sexual maturation enabling productive gametogenesis. mRNA profiles of neonatal wild type (WT) mice testis were generated by deep sequencing using Illumina HiSeq 2000 Examination of 2 different histone modifications in mouse spermatogonia Please note that ChIPSeq_Kitplus samples are samples isolated with MACS CD117 microbeads from Miltenyi and ChIPSeq_Kitminus are samples that were not positively selected for Kit.

ORGANISM(S): Mus musculus

SUBMITTER: Diana Low

PROVIDER: E-GEOD-62355 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription and imprinting dynamics in developing postnatal male germline stem cells.

Hammoud Saher Sue SS Low Diana H P DH Yi Chongil C Lee Chee Leng CL Oatley Jon M JM Payne Christopher J CJ Carrell Douglas T DT Guccione Ernesto E Cairns Bradley R BR

Genes & development 20151101 21

Postnatal spermatogonial stem cells (SSCs) progress through proliferative and developmental stages to populate the testicular niche prior to productive spermatogenesis. To better understand, we conducted extensive genomic profiling at multiple postnatal stages on subpopulations enriched for particular markers (THY1, KIT, OCT4, ID4, or GFRa1). Overall, our profiles suggest three broad populations of spermatogonia in juveniles: (1) epithelial-like spermatogonia (THY1(+); high OCT4, ID4, and GFRa1) ...[more]

PMID: 26545815

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