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Regulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells (III)

ABSTRACT: The goal is to identify RNAs that bind to Hnrnpl (hnRNP L) using RNA immunoprecipitation (RIP) assay. p53+/+ mES cells and p53-/- mES cells were untreated or treated with 0.5 uM Adriamycin for 8 hours. RNA immunoprecipitation was performed using Hnrnpl antibody (Bethyl, Cat#: A311-423A). Total RNA was extracted and subject to RNAseq.

ORGANISM(S): Mus musculus

SUBMITTER: Jing Huang

PROVIDER: E-GEOD-62378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An Apela RNA-Containing Negative Feedback Loop Regulates p53-Mediated Apoptosis in Embryonic Stem Cells.

Li Mangmang M Gou Hongfeng H Tripathi Brajendra K BK Huang Jing J Jiang Shunlin S Dubois Wendy W Waybright Tim T Lei Ming M Shi Jianxin J Zhou Ming M Huang Jing J

Cell stem cell 20150430 6

Maintaining genomic integrity is of paramount importance to embryonic stem cells (ESCs), as mutations are readily propagated to daughter cells. ESCs display hypersensitivity to DNA damage-induced apoptosis (DIA) to prevent such propagation, although the molecular mechanisms underlying this apoptotic response are unclear. Here, we report that the regulatory RNA Apela positively regulates p53-mediated DIA. Apela is highly expressed in mouse ESCs and is repressed by p53 activation, and Apela deplet ...[more]

PMID: 25936916

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