Unknown,Transcriptomics,Genomics,Proteomics

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Rare Allele of A Novel Histone H4 Acetyltransferase Enhances Grain Weight, Yield and Plant Biomass Presumably Through the Regulation of Transcription


ABSTRACT: Grain weight is an important crop yield component; however, its underlying regulatory mechanisms are largely unknown. Here, we identify a grain-weight quantitative trait locus (QTL) encoding a new-type GNAT-like protein that harbors intrinsic histone acetyltransferase activity (OsglHAT1). Our genetic and molecular evidences pinpointed the QTL-OsglHAT1’s allelic variations to a 1.2-kilobase region upstream of the gene body, which is consistent with its function as a positive regulator of the traits. Elevated OsglHAT1 expression enhances grain weight and yield by enlarging spikelet hulls via increasing cell number and accelerating grain filling, and increases global acetylation levels of histone H4. OsglHAT1 localizes to the nucleus, where it likely functions through the regulation of transcription. Despite its positive agronomical effects on grain weight, yield and plant biomass, the rare allele elevating OsglHAT1 expression has so far escaped human selection. Our findings reveal the first example, to our knowledge, of a QTL for a yield component trait being due to a chromatin modifier that has the potential to improve crop high-yield breeding. Two replicates of Nipponbare as control, three replicates of GW6a-4.6 substitution lines and three replicates of OsglHAT1-OE transgenic lines.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Tomoyuki Furuta 

PROVIDER: E-GEOD-62554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice.

Song Xian Jun XJ   Kuroha Takeshi T   Ayano Madoka M   Furuta Tomoyuki T   Nagai Keisuke K   Komeda Norio N   Segami Shuhei S   Miura Kotaro K   Ogawa Daisuke D   Kamura Takumi T   Suzuki Takamasa T   Higashiyama Tetsuya T   Yamasaki Masanori M   Mori Hitoshi H   Inukai Yoshiaki Y   Wu Jianzhong J   Kitano Hidemi H   Sakakibara Hitoshi H   Jacobsen Steven E SE   Ashikari Motoyuki M  

Proceedings of the National Academy of Sciences of the United States of America 20141222 1


Grain weight is an important crop yield component; however, its underlying regulatory mechanisms are largely unknown. Here, we identify a grain-weight quantitative trait locus (QTL) encoding a new-type GNAT-like protein that harbors intrinsic histone acetyltransferase activity (OsglHAT1). Our genetic and molecular evidences pinpointed the QTL-OsglHAT1's allelic variations to a 1.2-kb region upstream of the gene body, which is consistent with its function as a positive regulator of the traits. El  ...[more]

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