Expression study between dental pulp cells from TRPC6-mut individual and control individuals
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ABSTRACT: As TRPC6 channel induces CREB-mediated trancription, Dental pulp cells from TRPC6-mut patient and from 6 controls were analyzed in order to verify if the disruption of TRPC6 leads to transcriptional changes. Cells were grown until reach 80-90% of confluency in DMEM/F12 media supplemented with 15% fetal bovine serum (Hyclone, TX), 1% penicillin/streptomycin, and 1% non-essential amino acids and maintained under standard conditions (37°C, 5% CO2). RNAs samples were extracted from cells in passage 5 to 7.
ORGANISM(S): Homo sapiens
SUBMITTER: Alysson Muotri
PROVIDER: E-GEOD-62620 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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