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Expression study of human embryonic stem cells, dental pulp cells (DPCs) and induced pluripotent stem cells (iPSC) obtained from DPC for characterization of iPSC

ABSTRACT: iPSC were obtained from DPC from TRPC6-mut patient, a idiopathic autistic patient and a control. Original DPC and iPSC obtained were submited to expression analysis in order to check if the expression pattern obtained for the iPSC cells were closer related to embyonic cells than to the original DPC DPCs were grown until reach 80-90% of confluency in DMEM/F12 media supplemented with 15% fetal bovine serum (Hyclone, TX), 1% penicillin/streptomycin, and 1% non-essential amino acids and maintained under standard conditions (37°C, 5% CO2). RNA samples were extracted from cells in passage 5 to 7. iPSC were grown until reaches 70% of confluency in mTSER medium (Stem Cells Technologies). RNA samples were extracted from cells in passage 12 to 15.

ORGANISM(S): Homo sapiens

SUBMITTER: Alysson Muotri

PROVIDER: E-GEOD-62622 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression study of human embryonic stem cells, dental pulp cells (DPCs) and induced pluripotent stem cells (iPSC) obtained from DPC for characterization of iPSC

Project description:iPSC were obtained from DPC from TRPC6-mut patient, a idiopathic autistic patient and a control. Original DPC and iPSC obtained were submited to expression analysis in order to check if the expression pattern obtained for the iPSC cells were closer related to embyonic cells than to the original DPC

2014-10-23 | GSE62622 | GEO

Expression study between dental pulp cells from TRPC6-mut individual and control individuals

Project description:As TRPC6 channel induces CREB-mediated trancription, Dental pulp cells from TRPC6-mut patient and from 6 controls were analyzed in order to verify if the disruption of TRPC6 leads to transcriptional changes. Cells were grown until reach 80-90% of confluency in DMEM/F12 media supplemented with 15% fetal bovine serum (Hyclone, TX), 1% penicillin/streptomycin, and 1% non-essential amino acids and maintained under standard conditions (37°C, 5% CO2). RNAs samples were extracted from cells in passage 5 to 7.

2014-10-23 | E-GEOD-62620 | biostudies-arrayexpress

Microarray global microRNA expression of donor cells, fibroblast-derived iPSC, and stromal-primed cord-blood iPSC

Project description:Global microRNA analysis of (a) human embryonic stem cells, (b) low passage episomal stromal-primed myeloid iPSC, (c) intermediate passage fibroblast iPSC, and (d) samples of corresponding donor cells.

2017-12-31 | GSE44423 | GEO

Affect of iPSC Passage Number on Neuronal Differentiation

Project description:Induced pluripotent stem cells (iPSCs) are a valuable resource for neurological disease-modeling and drug discovery, due to their ability to differentiate into neurons reflecting the genetics of the patient from which they are derived. iPSC-derived cultures, however, are highly variable due to differences in culture conditions. We investigated the effect of iPSC passage number on differentiation to optimize the generation of functional, mature sensory neurons (iPSC-dSNs). Three iPSC lines were differentiated into iPSC-dSNs at passage numbers within each of the following ranges: low (LP; 5-10), middle (MP; 20-26), and high (HP; 30-38). Morphology and pluripotency of the parent iPSCs were assessed prior to differentiation at each passage number. iPSC-dSNs were evaluated based on electrophysiological properties and expression of key neuronal markers. All iPSC lines displayed the same morphology and were similarly pluripotent across passage numbers. iPSC-dSNs were also morphologically comparable across passage numbers. However, the expression levels of neuronal markers and an analysis of sodium channel function indicated greater maturity in LP iPSC-dSNs. Our results demonstrate that lower passage numbers may be better suited for differentiation into peripheral sensory neurons. Further studies are warranted to elucidate factors that may contribute to the variability associated with iPSC passage number.

2022-07-19 | GSE193571 | GEO

Profiling of iPSC-derived keratinocytes

Project description:Our goal was to demonstrate the similarity between the original keratinocytes and iPSC-derived keratinocytes from the same individual Profiles of original keratinocytes, iPSCs, IPSC-derived keratinocytes, iPSC-derived fibroblasts from a single individual and normal fibroblasts from a second individual were compared

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Global gene expression analysis of donor cells, low passage episomal stromal-primed myeloid iPSC, and intermediate passage fibroblast iPSC.

Project description:Global gene expression analysis of (a) human embryonic stem cells, (b) low passage episomal stromal-primed myeloid iPSC, (c) intermediate passage fibroblast iPSC, and (d) samples of corresponding donor cells.

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MicroRNAs Contribute to iPSC-Somatic Donor Memory

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Global gene expression analysis of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state

Project description:Global gene expression analysis of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state

2016-09-13 | GSE65211 | GEO

Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state

Project description:Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state

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