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Gene expression induced in HEK293 by treatment with the differentiating agents all-trans retinoic acid (tRA) and N6,2'-O-dibutyryl-cAMP (cAMP)

ABSTRACT: This gene array analysis shows that the transcriptome of HEK293, representing low-differentiated human embryonic kidney cells, cultured in presence of tRA and cAMP differentiating agents, is consistent with a genetic program relevant to kidney development Comparison of HEK293 cells grown in standard contitions or in presence of differentiating agents. Cells were seeded in 10 cm dishes, cultured for 3 days either in complete medium or in medium supplemented with tRA and cAMP, harvested, and spun down before RNA isolation. Comparative analysis allowed us to identify genes transcriptionally by tRA/cAMP.

ORGANISM(S): Homo sapiens

SUBMITTER: Loris De Cecco 

PROVIDER: E-GEOD-62722 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Retina-derived POU domain factor 1 coordinates expression of genes relevant to renal and neuronal development.

Fiorino Antonio A   Manenti Giacomo G   Gamba Beatrice B   Bucci Gabriele G   De Cecco Loris L   Sardella Michele M   Buscemi Giacomo G   Ciceri Sara S   Radice Maria T MT   Radice Paolo P   Perotti Daniela D  

The international journal of biochemistry & cell biology 20160715

Retina-derived POU domain Factor 1 (RPF-1), a member of POU transcription factor family, is encoded by POU6F2 gene, addressed by interstitial deletions at chromosome 7p14 in Wilms tumor (WT). Its expression has been detected in developing kidney and nervous system, suggesting an early role for this gene in regulating development of these organs. To investigate into its functions and determine its role in transcriptional regulation, we generated an inducible stable transfectant from HEK293 cells.  ...[more]

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